目的:研究产自云南省香格里拉地区的腺叶香茶菜(Isodon adenolomus)地上部分的化学成分;方法:利用正反相硅胶、高效液相色谱等现代分离技术进行分离;通过IR,UV,MS,NMR等波谱方法鉴定了化合物结构式;对化合物1进行了单晶X-衍射分析,确定了其绝对构型;化合物2-5和10进行了细胞毒活性测定;结果:分离得到7个对映-贝壳杉烷二萜,1个对映-松香烷二萜和两个松香烷二萜;结论:Isoadenolins M-O(1-3)为新化合物,且化合物2-4和10表现出了一定细胞毒活性。
AIM:To study the chemical constituents of the aerial parts of Isodon adennolmus collected in the Shangrila region of Yunnan Province,China.METHOD:Compounds 1-10 were separated and purified by silica gel and C18 reversed phase silica gel column chromatography.The structures were elucidated by extensive spectroscopic methods(IR,UV,MS and NMR),and the absolute configuration of 1 was determined by single crystal X-ray diffraction analysis.Compounds 2-5 and 10 were evaluated for their in vitro cytotoxicity against human tumor cell lines,HL-60,SMMC-7721,A-549,MCF-7,and SW-480.RESULTS:One ent-abietane,two abietane,and seven ent-kaurane diterpenoids were isolated and identified.CONCLUSION:Compounds 1-3 are new diterpenoids.Compounds 2-4 and 10 showed moderate inhibitory effects on several of the five tumor cell lines.