中文摘要：

目的：探讨蛋白酶体抑制剂硼替佐米（万珂，Valcade）对白血病K562细胞株细胞间粘附分子（ICAM-1）表达的影响。方法：用含10％FBS的RPMI1640培养液体外培养K562细胞，分别用0、10、20、30、50、100nmol／L的硼替佐米干预K562细胞6h后收集，用反转录聚合酶链反应（RT—PCR）分析K562细胞ICAM-1表达的变化。并用20nmol／L浓度硼替佐米作用K562细胞株不同时间（0，6，12，24，48h）分析K562细胞ICAM-1表达的变化。结果：硼替佐米明显抑制K562细胞ICAM-1的表达（P〈0,05），在硼替佐米浓度为0～20nmol／L时成正比关系，当浓度〉20nmol／L各组差异无显著性。以20nmol／L浓度作用K562细胞株不同时间后，ICAM-1的表达较作用前明显下降，并且这种效应持续存在。结论：K562细胞株ICAM-1基因表达异常增高，硼替佐米可抑制其表达量。

英文摘要：

Objective：To investigate the effects of proteasome inhibitor bortezomib （Valcade,PS-341） on the expression of intercellular adhesion molecule-1 （ICAM-1） of K562 cells. Methods： K562 cells were incubated with RPMI1640 and exposed to bortezomib in different concentration （0,10,20,30,50,100 nmol/L） and different time（0,6,12, 24,48 h）respectively. Then the expression of ICAM-1 was detected by RT-PCR. Results ：The expression of ICAM-1 was decreased significantly after bortezomib treatment. The inhibitory effect on ICAM-1 was positively related with drug concentration when the drug concentration was increased from 0 nmol/L to 20 nmol/L,whereas it remained unchangeable when the drug concentration exceeded 20 nmol/L. And another results showed that the inhibitory effect was lasted for 48 h at least. Conclusion：The expression of ICAM-1 in K562 cells is increased ,which can be inhibited by bortezomib.