本文采取宝生物公司RNAisoplus试剂从宽口裂腹鱼肌肉、脑、肾脏、肝脏、肠、性腺、心脏中提取总RNA,分析了所提宽口裂腹鱼不同组织总RNA的质量高低,及造成质量不同的具体原因,旨在为其他科研工作者在分析RNA质量时提供基础理论依据。
The total RNA of Schizothorax eurystomus(Kessler) from muscle,brain,kidney,liver,intestine,gonad and heart was extracted by RNAiso plus.The total RNA extracted from different tissues was quantified and compared,and aiming to find the cause of the quantities' variation.This study provides fundamental information on quantifying total RNA for other researchers.