中文摘要：

对比甲醇-盐酸混合物及硫酸处理对场效应器件（Field-effect device，FED）电化学测试性能的影响。实验结果表明，FED表面经过两种方式处理，其电化学阻抗测试特性发生变化，表现在阻抗的虚部值（用约表示）随加在FED器件与参比电极之间的电压的变化特性（用Zq-V曲线表示）发生改变。经过硫酸处理的器件的Zq-V曲线相对于未处理器件有微小偏移；而甲醇-盐酸体系处理后曲线在耗尽层区的斜率有所下降，表明这种处理对器件测试性能有负向的影响。经硅烷化固定在表面的氨基采用荧光标记，荧光测试结果表明，两种表面处理方式对硅烷化效果影响不大。进一步通过共价连接在硅烷化FED表面引入探针DNA分子，制备基于FED的DNA传感器。与目标DNA分子杂交反应后，实验表明，硫酸处理的测试结果要好于甲醇-盐酸的处理方法。

英文摘要：

The surface modification was carried out employing sulfuric acid and methanol-HCl solution respectively. The test characteristics were illustrated by the measurement of the out-phase impedance （referred to as Zq） vs bias voltage imposed between the FED and the reference electrode. The Zq-V curves are different after different surface modifications. With the sulfuric acid treatment, the Zq-V curve changes lit- tle, while the treatment by the methanol-HCl solution will cause the decrease of the sensitivity of the FED. The silanization and fluorescin-labeling of the surface after these two kinds of treatment reveal that the treatments cause little influence on the silanization effect of the surface. The FED-based DNA sensors were prepared after the surface modification of the FED. The methanol-HCl treatment will make the read- out of the DNA sensor decreased compared with the sulfuric acid treatment.