中文摘要：

这篇论文论述一杆石英水晶微量天秤(QCM ) 的开发为 E 的即时察觉的生物传感器。关口 i 01 57:H7 DNA 基于 nanogold 粒子扩大。许多内部 Au nanoparticles 被使不能调动到 Au 电极的 thioled 表面上，然后，搁浅单人赛的 DNA (ssDNA ) 探查的更特定的 thiolated 能通过 Au 嘘结合被修理。杂交被暴露 ssDNA 探查到 E 的互补目标 DNA 导致。关口 i 0157:H7 基因 eaeA，然后导致了一个集体变化和 QCM 的相应频率移动(Af ) 。外部卵白素涂的 Au nanoparticles 能与目标 DNA 结合增加团。电化学技术，循环伏安法(CV ) 和电气化学的阻抗光谱学(EIS ) 被采用表明并且特性每步。相应于 2.0 x 10 的目标 DNA (3 ) 殖民地形成单位(CFU )/mL E。关口 i 0157:1-117 房间能被这 blosensor 检测，因此基于特定的 DNA 分析为病原的细菌察觉开发一个敏感、有效的 QCM 生物传感器是实际的。压电的 blosensing 系统为进一步的应用程序有潜力，例如监视的食物安全和环境，并且这条途径为为内场细菌察觉把方法合并到一个综合系统放地基。

英文摘要：

This paper presents development of a quartz crystal microbalance （QCM） biosensor for real-time detection of E. coil O157：H7 DNA based on nanogold particles amplification. Many inner Au nanoparticles were immobilized onto the thioled surface of the Au electrode, then more specific thiolated sin- gle-stranded DNA （ssDNA） probes could be fixed through Au-SH bonding. The hybridization was induced by exposing the ssDNA probe to the complementary target DNA of E. coli O157：H7 gene eaeA, then resulted in a mass change and corresponding frequency shifts （ △f ） of the QCM. The outer avidin-coated Au nanoparticles could combine with the target DNA to increase the mass. The electrochemical techniques, cyclic voltammetry （CV） and electrochemical impedance spectroscopy （EIS） were adopted to manifest and character each step. The target DNA corresponding to 2.0×10^3 colony forming unit （CFU）/mL E. coil O157：H7 cells can be detected by this biosensor, so it is practical to develop a sensitive and effective QCM biosensor for pathogenic bacteria detection based on specific DNA analysis. The piezoelectric biosensing system has potential for further applications, such as food safety and environment monitoring, and this approach lays the groundwork for incorporating the method into an integrated system for in-field bacteria detection.