中文摘要：

脂联素主要是由脂肪细胞分泌的一种脂肪细胞因子，对啮齿类动物的研究发现，脂联素通过垂体细胞存在的脂联素受体，对LH的分泌起抑制作用，从而影响繁殖功能。本实验旨在了解脂联素受体mRNA与生殖相关因子mRNA在皖南花猪垂体组织中表达的发育性变化及相关性。采用荧光实时定量PCR方法检测0、30、45、90和180d（各日龄样本数10头，公母各5头）皖南花猪垂体中脂联素受体（AdpR1和AdpR2）、促性腺激素释放激素受体（GnRHR）、黄体生成素（CH-β）、卵泡刺激素（FSH-β）mRNA的表达水平，ELISA方法检测血液中LH与FSH的浓度变化。结果表明：（1）垂体AdpR2mRNA有显著发育性变化（Jp〈0．05）；A咖R，mRNA各个日龄的表达均显著高于A咖R2mRNA，公猪在0、30和180d显著高于母猪；（2）LH-β与FSH-βmRNA有显著发育性变化（P〈0．05），且母猪FSH-β在0d显著高于公猪（P〈0．05），公猪GnRHRmRNA在90d显著高于母猪（P〈0．05）。血液中LH和FSH有极显著的发育性变化（P〈0．01），母猪各个日龄耶Ⅳ含量均高于公猪。（3）公猪／4咖R1mRNA分别与G，tRHR、FSH-β、LH-βmRNA负相关（P〈0．05），A咖R2mRNA与GnRHRmRNA负相关（P〈0．05）；母猪f4咖尺2与GnRHR、LH-β、血清FSH分别负相关（P〈0．01或P〈0．05）；此外，公、母猪AdpR1与AdpR2 mRNA均正相关（P〈0．01或P〈0．05），GnRHR分别与FSH-β和LH-[3mRNA正相关（P〈0．01或P〈0．05）。本研究结果提示，脂联素可能经AdpR介导，通过GnRHR，或直接对垂体FSH和LH的分泌起抑制调节作用。

英文摘要：

Adiponectin（Adp） is an adipocytokine maily secreted by adipocytes. Evidence suggests adiponectin action on the reproductive system. Recent researches in roden showed that Adp inhibits LH secretion mediated by AdpR in pituitary. The aims of this study were to observe the correlation and developmental patterns of adiponectin receptors and reproduction-related factors mRNA expressions in pituitary of Wannahua pigs. The test animals were sampled on 0, 30, 45, 90, and 180 d of age, including 5 boars and 5 sows at each different days of age The development patterns of adiponectin receptors（AdpR1, AdpR2）, Gonadotropin Releasing Hormone Receptor（GnRHR）, Luteinizing Hormone（LH-/3）, and Follicle Stimulating Hormone（FSH-β） mRNA in pituitary were investigated and their correlation were analyzed by Real-time fluorescent quantitative PCR（RT-PCR） while the concentration of LH and FSH in serum at different days of age were detected by ELISA. The results showed that： 1） The expression of AdpR2 mRNA were significantly changed with age increasing（P〈0.05）; The mRNA expression of AdpR1 in different days were significantly higher than that of AdpR2 at any days of age, while the expression of AdpR1 at birth, 30 and 180 d of boars were significantly higher than that of those of sows. 2） The expression of LH-β at different growth stages and FSH-β mRNA in different days of sows were significantly changed with age increasing（P〈0.05）; The expression of FSH-β at birth of sows was significantly higher than that of boars（P〈0.05） and GnRHR at 90 d of boars was significantly higher than that of sows（P〈0.05）. The LH and FSH level in serum were significantly changed with age increasing（P〈0.01）. The FSH contents in different days of sows were higher than those of boars. 3）The expression of AdpR1 in different days of boars were negatively correlated with GnRHR, FSH-β and LH-β respectively（P〈0.05）. The expression of AdpR2 of boars were negatively correlated with GnRHR（P〈0.0