中文摘要：

旨在了解皖南花猪睾丸组织脂联素受体mRNA与睾酮合成相关因子mRNA表达的发育性变化及其相关性,探讨脂联素与睾酮生成的关系。本研究用荧光实时定量PCR法检测0、30、45、90和180d等不同日龄（各日龄样本数5头）皖南花猪睾丸组织脂联素受体（AdpR1、AdpR2）和LHR、CYP11A1、StARmRNA的表达水平。结果,AdpR1、CYP11A1和StARmRNA的表达有极显著的发育性变化（P〈0.01）,AdpR1mRNA的表达先增加后降低,45d达到最大值;CYP11A1和StAR mRNA的表达先升高后降低再升高,45d达到最大表达量。LHR mRNA的表达也有显著的发育性变化（P〈0.05）,整体表现为先升高后降低。AdpR1与CYP11A1mRNA的表达量显著相关（r=0.587,P〈0.05）;LHR与CYP11A1、StAR mRNA的表达量分别显著相关（r=0.528,P〈0.05;r=0.552,P〈0.05）;CYP11A1与StARmRNA表达量极显著相关（r=0.709,P〈0.01）。AdpR1mRNA在睾丸组织的高表达提示脂联素可能通过AdpR1介导对睾丸的调节作用,其发育性变化和CYP11A1mRNA呈正相关,提示脂联素对睾酮的生成有一定的促进作用。

英文摘要：

The aims of this study were to observe the developmental patterns and the correlation of adiponectin receptors and testosterone synthesis-related factors mRNA expressions in testis of Wannan Hua pig.To investigate the relationship of adiponectin with the generation of testosterone.Five Wannan Hua boars were sampled at birth,30,45,90,and 180 days of age,respectively.The expression of AdpR1,AdpR2,LHR,CYP11A1 and StAR mRNA in testis were detected by real-time fluorescence quantitative RT-PCR.The expression of AdpR1,CYP11A1 and StAR mRNA were significantly changed with age increasing（P〈0.01）.The expression of AdpR1 mRNA increased at first and then decreased with age increasing,while CYP11A1 and StAR mRNA showed an increasing at first,then decreasing,and increasing at last.All of their expression increased to the top at 45 days of age.LHR were also significantly changed with age increasing（P〈0.05）,which increased at first then decreased.Significant positive correlation was found between mRNA expression of AdpR1 and CYP11A1（r=0.587,P〈0.05）.The mRNA expression of LHR were significantly correlated with CYP11A1（r=0.528,P〈0.05） and StAR（r=0.552,P〈0.05）,respectively.And significant positive correlation was found between CYP11A1 and StAR mRNA expression（r=0.709,P〈0.01）.The high expression of AdpR1 mRNA in testis suggested that the action of adiponectin on testis may mediated by AdpR1.The positive correlation between AdpR1 and CYP11A1 mRNA expression indicated that adiponectin contribute the generation of testosterone.