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不同物料堆肥腐熟度评价指标的变化特性
  • ISSN号:1001-6929
  • 期刊名称:环境科学研究
  • 时间:2014.6.15
  • 页码:623-627
  • 分类:Q178.513[生物学—水生生物学;生物学—普通生物学] X53[环境科学与工程—环境工程]
  • 作者机构:[1]College of Life Science, Northeast Agricultural University, Harbin 150030, China
  • 相关基金:Supported by the National Natural Science Foundation of China (No. 51178090), the National Key Technology R&D Program of China (No. 2012BAJ21B02-02), and the National Water Pollution Control and Management Technology Major Projects (No. 2009ZX07106-001)
  • 相关项目:荧光光谱特性表征堆肥腐熟程度应用研究
中文摘要:

在深人工的湖松胡亚·莱克,探索浮游生物社区的空间异质中国,样品在七个地点被收集。样品被使中毒调查放大 PCR 的 16S 和 18S rRNA 基因和特定的乐队的坡度胶化电气泳动 DGGE 分析被定序。DGGE 侧面的簇分析表明所有样品组织了进二不同的簇,根据采样地点;当时在每簇,亚簇的分叉与采样深度相关。选择主导的 DGGE 乐队的顺序分析表明大多数定序 84% 展出了的 phylotypes 97% 类似到在 GenBank 的最靠近的序列,并且隶属于十普通淡水浮游生物数 Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles,和 Rotifera。几个这些组也被发现世界范围,显示 phylotypes 的大都会的分发。在 DGGE 模式和环境因素之间的关系被冗余性分析 RDA 分析。建议的结果那,全部的氮,硝酸盐,亚硝酸根,氨,和货到付款 < 潜水艇 class= “ a-plus-plus ” > Mn 集中,和水温度强烈在浮游生物作文与变化被相关。

英文摘要:

To explore the spatial heterogeneity of plankton communities in a deep artificial lake (Songhua Lake, China), samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis (DGGE) analysis of the PCR-amplified 16S and 18S rRNA genes and specific bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters, in accordance with sampling site; while in each cluster, the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes (84%) exhibited 〉97% similarity to the closest sequences in GenBank, and were affiliated with ten common freshwater plankton phyla (Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles, and Rotifera). Several of these groups are also found worldwide, indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis (RDA). The results suggested that, total nitrogen, nitrate, nitrite, temperature were strongly correlated with the variation ammonia, and CODMn concentrations, and water in plankton composition.

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期刊信息
  • 《环境科学研究》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国环境保护部
  • 主办单位:中国环境科学研究院
  • 主编:刘鸿亮
  • 地址:北京安外大羊坊8号中国环科院内
  • 邮编:100012
  • 邮箱:hjkxyj@163.com
  • 电话:010-84915128
  • 国际标准刊号:ISSN:1001-6929
  • 国内统一刊号:ISSN:11-1827/X
  • 邮发代号:82-384
  • 获奖情况:
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,荷兰文摘与引文数据库,美国工程索引,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国国家哲学社会科学学术期刊数据库,中国北大核心期刊(2000版)
  • 被引量:33433