在深人工的湖松胡亚·莱克,探索浮游生物社区的空间异质中国,样品在七个地点被收集。样品被使中毒调查放大 PCR 的 16S 和 18S rRNA 基因和特定的乐队的坡度胶化电气泳动 DGGE 分析被定序。DGGE 侧面的簇分析表明所有样品组织了进二不同的簇,根据采样地点;当时在每簇,亚簇的分叉与采样深度相关。选择主导的 DGGE 乐队的顺序分析表明大多数定序 84% 展出了的 phylotypes 97% 类似到在 GenBank 的最靠近的序列,并且隶属于十普通淡水浮游生物数 Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles,和 Rotifera。几个这些组也被发现世界范围,显示 phylotypes 的大都会的分发。在 DGGE 模式和环境因素之间的关系被冗余性分析 RDA 分析。建议的结果那,全部的氮,硝酸盐,亚硝酸根,氨,和货到付款 < 潜水艇 class= “ a-plus-plus ” > Mn 集中,和水温度强烈在浮游生物作文与变化被相关。
To explore the spatial heterogeneity of plankton communities in a deep artificial lake (Songhua Lake, China), samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis (DGGE) analysis of the PCR-amplified 16S and 18S rRNA genes and specific bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters, in accordance with sampling site; while in each cluster, the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes (84%) exhibited 〉97% similarity to the closest sequences in GenBank, and were affiliated with ten common freshwater plankton phyla (Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles, and Rotifera). Several of these groups are also found worldwide, indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis (RDA). The results suggested that, total nitrogen, nitrate, nitrite, temperature were strongly correlated with the variation ammonia, and CODMn concentrations, and water in plankton composition.