中文摘要：

目的评价利奈唑胺（嗯唑烷酮类抗菌药）、万古霉素（胺基糖苷类抗生素）2种抗菌药物对耐甲氧西林金黄色葡萄球菌（MRSA）的体外抗菌活性。方法用琼脂二倍稀释法，测定抗菌药物的最低抑菌浓度（MIC）；用肉汤稀释法，测定抗菌药物的最低杀菌浓度（MBC），绘制杀菌曲线（KCs）。结果利奈唑胺对临床分离的98株MRSA的MIC50为1μg·mL^-1，MIC90为2μg·mL^-1，MBC50为8μg·mL^-1，MBC90为32μg·mL^-1，敏感率为100％；万古霉素对临床分离的98株MRSA的MIC50为1μg·mL^-1，MIC90为1μg·mL^-1，MBC50为8μg·mL^-1，MBC90为32μg·mL^-1，敏感率为100％。随着抗菌药物浓度的升高，其杀菌时间缩短不甚明显，呈现非浓度依赖性的特点。结论利奈唑胺对MRSA的体外抗菌活性与万古霉素相当，均显示非浓度依赖性的杀菌曲线。

英文摘要：

Objective To evaluated the in vitro antimicrobial activities of linezolid and vancomycin agents against methicillin -resistant Staphylococcus aureus （MRSA）. Methods The minimal inhibitory concentrations （MICs） of antibacterial agents were determined by the standard two -fold agar dilution method. The minimal bactericidal concentrations （MBCs） antibacterial agents were determined by tube broth dilution method and time -killing curves were obtained. Results The sensitive rates to linezolid and vancomycin against MRSA were both 100%, the MIC50 and MIC90 were 1 μg·mL^-1,1 μg·mL^-1 and 2 μg·mL^-1, 1 μg·mL^-1. 32 μg·mL^-1 respectively. The MBC50 and MBC90 were both 8μg·mL^-1,32μg·mL^-1, respectively. With the increase of drug concentrations, the killing time did not show obvious decline. Conclusion Linezolid and vancomycin have strong antibacterial activity against MRSA, and they have nonconcentration independent characteristic.