中文摘要：

目的：研究片仔癀对人卵巢癌细胞株OVCAR-3增殖抑制作用，及其对细胞周期、细胞凋亡的影响。方法：采用MTT法观察片仔癀对人卵巢癌细胞株OVCAR-3细胞的增殖抑制率，流式细胞仪检测细胞凋亡及细胞周期，westem—blot检测相关蛋白的表达。结果：片仔癀以剂量依赖式抑制人卵巢癌细胞株OVCAR-3细胞增殖，片仔癀250、500、1000μg·mL-1作用于OVCAR-3细胞24h后，其早期凋亡率分别为6．6％、30．9％、43．2％，而对照组为0％，其诱导凋亡作用呈现剂量依赖性；细胞积聚在G0／G1期，同时S期细胞比例减少；Akt、PARP、CDK6表达下调。结论：片仔癀可以抑制OVCAR-3细胞增殖及诱导细胞凋亡作用，并能阻滞细胞于G0／G1期，有望成为卵巢癌治疗药。

英文摘要：

Objective： To explore the effects ofPien Tze Huang on the proliferation, apoptosis and cycle of human ovarian cancer cell line OVCAR-3. Methods： To observe the effect of Pien Tze Huang on the OVCAR - 3 cell proliferation inhibition rate by MTT method, to detect cell apoptosis and cycle by flow cytometry instrument detect the protein expression by western blot. Results： Pien Tze Huang in a dose dependent type inhibits the proliferation of human ovarian cancer cell line OVCAR-3 cells, Pien Tze Huang 250, 500, 1000μg·mL-1 acting on OVCAR-3 cells after 24 h, the early apoptosis rate were 6.6%, 30.9%, 43.2%, and 0% for the control group, the apoptosis is dose dependent; cell accumulation is in G0/G1 period, and the percentage of cells in S phase is decreased; there is a lower expression of Akt, PARP, CDK6. Conclusion： Pien Tze Huang can inhibit and induce the apoptosis of OVCAR-3 cell proliferation, and can block the cell in G0/G1 phase, it is expected to become a drug for the treatment of ovarian cancer.