中文摘要：

目的：克隆和分析油茶高亲和磷转运蛋白基因，为研究其结构和功能打下基础。方法：以油茶品种‘华硕’为试材，通过RT—PCR和RACE的方法克隆出油茶磷酸转运子Phtl基因家族一个成员的全长cDNA序列，命名为CoPhtl；2（GenBank登录号：JX412956．1），通过生物信息学技术对其序列的理化性质、结构与功能进行分析和预测。结果：CoPhtl；2CDS长度为1590bp，编码530个氨基酸，与其它物种的Phtl氨基酸序列具有较高的相似性，其中与杨柳科毛果杨的Phtl相似性最高，达到77．5％；该基因所编码蛋白质的分子量为58．02kDa，理论等电点pI为8．97，二级结构主要由a-螺旋、p-折叠和不规则卷曲构成，包含12个明显的跨膜螺旋拓扑结构。结论：预测显示该蛋白是一个疏水跨膜蛋白，具有磷转运蛋白的主要特征，初步判定其与油茶磷吸收有关，其功能有待进一步验证。

英文摘要：

Objective： To provide a basis for structure and function＇ s research of Phtl in oihea. Method： The phosphate transporter named CoPhtl ;2 was isolated from oil tea（ Camellia oleifera ＇ Huashuo＇ ）with the method of RT -PCR and RACE. Bioinformatics approach was used to analyze and predict the function and domain of CoPhtl ; 2. Result：The CDS of CoPhtl ; 2 is 1 590 bp in length and it codes a pre- dicted protein of 530 amino acids. The amino acid identity compared with other phosphate transporters is highly conserved and showed the highest similarity（77. 5% ）with the CoPhtl ;1 of Populus trichocarpa. The estimated molecular weight and isoelectric point of the putative protein were 58.02 kDa and 8. 97. Concluslon：Putative secondary structure and topology of the encoding protein have the main feature of transmembrane protein, and is consistent with Phtl encoding protein in other species.