接种稳定运行300余天的厌氧氨氧化污泥,通过批次试验,研究了不同浓度乙酸钠和不同种类有机物对厌氧氨氧化系统的冲击影响.结果表明:在初始NO2^--N浓度为35mg/L左右,乙酸钠浓度为0-200mg/L时,乙酸钠的冲击不会抑制厌氧氨氧化菌的活性,且一定程度上促进厌氧氨氧化反应的进行,最大比氨氧化速率与乙酸钠浓度呈正相关性;不同有机物对厌氧氨氧化系统的促进作用不同,氨氧化速率从高到低依次为乙酸钠、蛋白胨、葡萄糖和淀粉;反硝化作用伴随整个反应过程,但硝态氮还原速率[0.0155-0.0442mgN/(L·min)J小于氨氧化速率[0.1090-0.1498mgN/(L·min)],因此厌氧氨氧化菌在系统中一直占主导地位.在有机物的冲击下,厌氧氨氧化反应可协同反硝化反应去除系统中的总氮,提高系统总氮的去除率,从而改善出水水质.
Bath experiments were conducted to investigate the shock effect of different concentration of sodium acetate and kinds of organic matters on anaerobic ammonia oxidation (Anammox) system, with the inoculation sludge derived from a stable Anammox system for more than 300 days. The results showed that under the initial nitrite concentration of 35mg/L and sodium acetate concentration below 200mg/L, anammox bacteria activity was not inhibited,but was promoted. The maximum specific ammonia oxidation rate increased with an increase in sodium acetate concentration. For the bath experiments of different organic matters, the maximum ammonia oxidation rate was observed in the Anammox system fed with sodium acetate, followed by peptone, glucose and starch. Denitrification was also associated with the Anammox, but nitrate reduction rate [0.0155-0.0442mgN/(L.min)] was lower than ammonia oxidation rate [0.1090-0.1498mgN/(L.min)], which illustrated that Anamrpox bacteria played a dominant role. Under the shock loading of organic matters, Anammox and denitrification could coordinately remove nitrogen compound, and enhance the total nitrogen removal efficiency, which made the effluent quality to improve to some extent.