中文摘要：

参考GenBank中收录的金黄色葡萄球菌FnBPA（fibronectin-bindingproteinA）基因和牛IFN-7序列进行了信号肽序列的分析。分析结果显示，牛IFN-7信号肽序列相关指标优于金黄色葡萄球菌FnBPA基因自身的信号肽序列。在此基础上利用重叠延伸PCR法以牛IFN-7信号肽序列取代金黄色葡萄球菌FnBPA基因的信号肽序列，并构建分泌型真核表达载体PVSFn。该重组表达载体转染BHK-21细胞后经ELISA及Western-blot检测证实可有效分泌表达目的蛋白。

英文摘要：

In order to construct eukaryotic expression plasmid of bovine Staphylococcus aureus fibronec- tin-binding protein A （FnBPA） and increase its expression efficacy in eukaryotic cells, sequence analysis was conducted on different signal peptides. Based on the sequences published by Genbank, two signal pep- tide sequences of S. aureus FnBPA and bovine interferon-）＇ （IFN-）＇） were compared and analyzed. Our re- sults indicated that bovine IFN-）＇ signal peptide sequence was better than that of S. aureus. On the basis of which,the signal peptide of S. aureus FnBPA was replaced by that of bovine IFN-y with duplication and extension method,then eukaryotic expression vehicle PV-SFn was constructed. This recombinant plasmid was used to transfect BHK-21 cell and ELISA assay and Western-blot results indicated that it was able to express and secrete our target protein effectively.