中文摘要：

采集新疆奶牛隐性乳腺炎乳样，对分离鉴定的致病性金黄色葡萄球菌，提取分离菌株的基因组DNA，参考GenBank中发表的ClfA（Clumping Factor A of Staphylococcus aureus）基因序列，设计特异性引物经PCR扩增得到1560bp的ClfA基因。对新疆3个地区分离的9株金黄色葡萄球菌分别进行ClfA基因的克隆和序列分析，对ClfA蛋白的氨基酸序列分析结果表明，新疆3个地区ClfA蛋白氨基酸的同源性为91．5％～100％，与国外其他分离菌株的同源性为91．9％～100％，差异性较小，该结果表明ClfA基因在该菌进化和流行过程中是保守的。

英文摘要：

Staphylococcus aureus which caused subclinical mastitis in bovine was isolated from Xinjiang bovine milk and identified.According to the ClfA gene sequences published by GenBank,a pair of specific primers were designed.ClfA gene was amplified by PCR with genomic DNA and the CIfA gene fragment was 1560 bp. This gene fragment was cloned and gene was analyzed by sequencing. Our result showed that the amino acid homology of CIfA protein for 3 regions in Xinjiang area was 91.5%-100%,compared with other countris the amino acid homology of ClfA protein was 91.9%-100%. These data indicated that the ClfA gene was conserved during the bacterial evolution and prevalence and provided experiment foundation for using ClfA to prevent infection caused by S. aureus in future.