中文摘要：

目的 观察胶原、生长因子刺激后肝星状细胞迁移变化,并观察生长因子刺激后细胞骨架的变化.方法 分离原代大鼠肝星状细胞,用Transwell小室观察Ⅰ型胶原、Ⅳ型胶原、血小板衍生生长因子-BB、转化生长因子β1直接及趋化刺激后细胞迁移改变,并按不同处理方式分为对照组、转化生长因子β1趋化刺激组、PDGF-BB趋化刺激组、Ⅰ型胶原趋化刺激组、Ⅳ型胶原趋化刺激组、转化生长因子β 1直接刺激组、PDGF-BB直接刺激组、Ⅰ型胶原直接刺激组、Ⅳ型胶原直接刺激组.异硫氰酸荧光素标记的鬼笔环肽标记F-肌动蛋白,共聚焦激光扫描显微镜观察血小板衍生生长因子-BB、转化生长因子β 1刺激后细胞骨架的变化.多组样本均数间差异比较用单因素方差分析.结果 对照组迁移细胞数为每高倍视野（102.93±1.01）个,转化生长因子β 1趋化刺激组及直接刺激组迁移的细胞数分别为每高倍视野（210.17±1.78）个、（131.37±3.15）个,与对照组相比,F值分别为40.84,64.53,P值均〈0.05,差异均有统计学意义; PDGF-BB趋化刺激组及直接刺激组迁移细胞数为每高倍视野（319.56±11.71）个、（203.67±7.54）个,与对照组相比,F值分别为40.90、54.57,P值均〈0.05,差异均有统计学意义;Ⅰ型胶原趋化刺激组及直接刺激组迁移细胞数为每高倍视野（201.52±11.28）个、（127.20±6.47）个,与对照组相比,F值分别为41.01、36.49,P值均〈0.05,差异均有统计学意义;Ⅳ型胶原趋化刺激组及直接刺激组迁移细胞数为每高倍视野（108.14±4.35）个、（108.60±4.10）个,与对照组相比,F值分别为4.94、5.42,P值均〉0.05,差异均无统计学意义.血小板衍生生长因子-BB及转化生长因子β1刺激后细胞形态改变,随时间不同出现层状伪足、丝状伪足及应力纤维的改变. 结论 Ⅰ型胶原、血小板衍生生长因子-BB、转化生长因子β1可促进肝星状细胞迁移,?

英文摘要：

Objective To evaluate the effects of collagens and growth factors TGF β1 and PDGFBB on the cytoskeletal components and migration in cultured rat hepatic stellate cells （HSCs）. Methods Primary rat hepatic stellate cells were isolated and cultured. A Transwell Chamber system was used to observe the changes of serum starved HSCs haptotactic migration （direct stimulation） and chemotactic migration （indirect stimulation） after collagens and growth factors treatment. Changes in actin cytoskeletal organization were visualized by fluorescence staining using FITC-labeled phalloidin and the fluorescence images were recorded using confocal microscopy. Results TGF β1 enhanced significantly the motility of primary HSCs at 5 ng/ml： for haptotactic migration cells： 131.37 ± 3.15 vs 102.93 ± 1.01, F = 40.84, P 〈 0.05; for chemotactic migration cells： 210.17 ± 1.78 vs 102.93 ± 1.01, F = 64.53, P 〈 0.05. PDGF-BB enhanced signifi cantly the motility of primary HSCs at 10 ng/ml （haptotactic migration cells： 203.67 ± 7.54 vs 102.93 ± 1.01,F= 40.90, P 〈 0.05; chemotactic migration cells： 319.56 ± 11.71 vs 102.93 ± 1.01, F = 54.57, P 〈 0.05）;Both chemotatic and haptotactic stimuli with 100 μg/ml collagen type（cn）significantly increased HSCs migration：for haptotactic migration cells： 127.20 ± 6.47 VS 102.93 ± 1.01, F = 41.01, P 〈 0.05; for chemotactic migration cells： 201.52 ± 11.28 vs 102.93 ± 1.01, F= 36.49, P 〈 0.05; however, collagen type Ⅳ had no effect on HSCs migration. Serum-starved, untreated cells had a rounded-up morphology. PDGF-BB and TGF β1 induced a rapid morphological change concomitant with a robust reorganization of actin cytoskeleton in HSCs. Conclusions Collagen type I, PDGF-BB and TGF βl could induce cell migration in HSCs, but collagen type Ⅳ has no such effect. PDGF-BB and TGF β1 could induce the actin cytoskeleton reorganization in HSCs.