中文摘要：

目的采用同步辐射光源和微血管成像技术对大鼠后肢的微循环进行成像观察。方法分别采用欧乃派克和硫酸钡作为对比剂，对大鼠后肢的微循环进行同步辐射活体吸收成像和离体相位衬度成像观察，并对图像进行三维重建。结果同步辐射微血管成像可清晰显示大鼠髂动脉的4级以上分支，活体和离体成像可观察到直径为40斗m和9斗m的血管，三维成像可显示和定位大鼠后肢中微米级别的微血管。结论应用同步辐射成像技术以及合适的对比剂可清晰显示大鼠后肢的微循环，为研究肢体微血管形态结构和功能提供了新的思路。

英文摘要：

Objective To detect deep-level microvascular structure in rat hind limb by synchrotron radiation and microangiographic technique. Methods Microangiography in vivo and ex vivo was performed by synchrotron radiation based absorption imaging and phase contrast imaging, with omnipaque and barium sulfate solution as contrast media, respectively, and synchrotron radiation-based micro-computed tomography （SRmCT） was also performed to reveal three-dimensional morphology of the blood vessel in rat hind limb. Results Using microangiographic technique in vivo and in vitro （with barium sulfate）, blood vessels in the rat limb muscle could be visualized with high resolution, and the fourth branches of iliac artery in rat hind limb could be detected with the minimum visualized blood vessels about 40 Ixm and 9 ~m in diameter, respectively. In addition, the vascular network could be defined and analyzed at the micrometer scale from the 3D renderings of limb vessel as shown by SRmCT. Conclusion Synchrotron radiation-based microangiography and SRmCT thus provided a practical and effective means to observe the microvasculature of rat hindlimb, which might be useful in assessment of angiogenesis in lower limbs.