以黑核桃内生真菌HJ1菌体为材料,研究了提取温度、提取时间和料液比对菌体油脂得率的影响。经单因素试验、正交试验及验证试验,结果表明:HJ1油脂得率为22.26%左右,最佳提取工艺条件为:提取温度85℃,提取时间4 h,料液比1∶6。HJ1油脂组成与植物油脂组成相似,可作为制备生物柴油的原料。
Endophyte HJ1 from black walnut was used to extract oil with petroleum.Extraction parameters were examined by single and orthogonal experiments.The yield of the oil from endophyte maintained around 22.26% under the optimal extraction conditions,i.e.,temperature: 85℃,extraction time: 4h,ratio of material to solvent: 1∶6.The constituents of the oil were similar with other plant oil,indicating that it can be used as raw material for biodiesel.