中文摘要：

目的：探讨Six1基因在宫颈癌组织中的表达情况及其临床意义。方法免疫组织化学SP法检测123例宫颈癌组织，49例宫颈上皮内瘤变（CIN）组织及32例正常宫颈组织中Six1蛋白的表达。体外培养人宫颈癌HeLa细胞，免疫荧光染色观察Six1蛋白在细胞中的定位。结果宫颈癌组织Six1蛋白阳性表达率（72.3%）高于CIN （28.6%）及正常宫颈组织（15.6%），χ^2分别为13.118，10.058（均P＜0.01）。不同肿瘤大小，淋巴结及血道转移情况的宫颈癌组织中Six1蛋白阳性表达率差异有统计学意义（均P＜0.01）。Six1蛋白在HeLa细胞中主要定位于细胞浆和核仁。结论 Six1与宫颈癌的发生发展密切相关，可能成为宫颈癌的侵袭与转移的分子标志物。

英文摘要：

Objective To investigate the clinical significance of protein expression of Six1 in cervical cancer. Meth-ods The immunohistochemical （IHC） staining was applied to detect the expression of Six1 protein in normal cervical tis-sues （n=32）, cervical intraepithelial neoplasia （CIN） tissues （n=49） and cervical cancer tissues （n=123）. The localization of Six1 protein was detected in vitro cultured HeLa cells using immunofluorescence （IF） staining. Results The positive rate of Six1was significantly higher in cervical cancer （72.3%） than that of CIN tissues （28.6%） and normal cervical tissues （15.6%,χ^2=13.118 and 10.058 respectively, P〈0.01）. There were significance differences in expression levels of Six1 protein be-tween different tumor sizes and metastasis of cervical cancer （P 〈0.01）. The Six1 protein showed positive signals in cyto-plasm and nucleoli in HeLa cells. Conclusion Six1 expression is associated with cervical cancer, which may be a potential biomarker for invasion and metastasis of cervical cancer.