中文摘要：

这研究报导了那幸运十 imidacloprid， 1-[(6-Carboxylethylthio-3- pyridinyl ) 甲基)-N-nitro-imidazolidinimine 用 imidacloprid 的技术材料被综合在热碱的答案与 3-mercaptopropionic 酸反应。幸运十被结合到牛的浆液白朊(BSA ) 和 ovalbumin (卵) 分别地形成免疫抗原和涂层抗原。有高效价的抗体(2.56 × 104 ) 在 immuning 兔子以后被获得。结果证明抗体有一种高亲密关系到连接酶的免疫吸着剂试金(ELISA ) 测试的 imidacloprid， IC50 和 IC20 是它 20.7 和 1.1 μ g L ? 1；并且到与 imidacloprid 联系的那些混合物的生气 reactibilities 是不到 1.4% 。

英文摘要：

This study reported that a hapten of imidacloprid, 1-[（6-Carboxylethylthio-3- pyridinyl）methyl]-N -nitro-imidazolidinimine was synthesized using technical material of imidacloprid to react with 3-mercaptopropionic acid in hot alkaline solution. The hapten was conjugated to bovine serum albumin （BSA） and ovalbumin （OVA） to form the immuno-antigens and the coating antigen, respectively. The antibody with high titer （2.56 × 10^4） was obtained after immuning rabbits. The results showed that the antibody had a high affinity to imidacloprid tested by enzyme-linked immunosorbent assay （ELISA）, which IC50 and IC20 were 20.7 and 1.1 μg L^-1; and the cross reactibilities to those compounds related with imidacloprid were less than 1.4%.