中文摘要：

目的研究核因子κB（NF-κB）抑制剂SN50对脂多糖（LPS）作用下小鼠巨噬细胞（Ana一1）炎性因子TNFdmRNA和蛋白表达的影响及其对NF—κBP6s亚基核移位的抑制作用。方法用MTT法检测SN50对细胞增殖活性的影响，行RT—PCR和ELISA法检测巨噬细胞经LPS作用和SN50处理后TNFαm—RNA和蛋白表达变化，用westem—Blot检测NF-κBP65亚基核移位情况。结果在LPS作用下炎性因子TNFoL强烈升高，并呈现时间依赖性，SN50干预后可明显抑制NF-κBp65亚基核移位和TNFα表达，其中以早期使用SN50效果最佳。结论SN50可以通过抑制NF—κBp65亚基核移位来降低LPS作用下巨噬细胞的TNFα的产生。

英文摘要：

[ Objective ] To investigate the effect of SN50, an inhibitor of nuclear factor κB （NF-κB）, on the TNFα production and on the activity of NF-κB in the mouse maerophage （Ana-1） stimulated by LPS. [Methods] Ana-1 cell＇s proliferation affected by SN50 was measured by MTT. The TNFot mRNA level was assayed by RT-PCR and the production of protein was measured by ELISA after being activated by LPS and being treated by SN50. The activity of NF-κB was assayed by Western-Blot. [ Results ] SN50 inhibited LPS-stimulated mRNA expression and production of TNFα in a eoneentration-dependent manner, as well as the activity of NF-κ. SN50 would do better if it was used as early as it could. [ Conclusion ] The inhibitory effect of SN50 on the production of TNFα in LPS- stimulated Ana-1 may be mediated by suppression of NF-κB activity.