中文摘要：

目的：探讨RACK1表达水平与食管鳞状细胞癌不同转移潜能细胞侵袭转移能力的关系。方法：Transwell实验检测食管鳞癌EC9706-H、EC9706-L和EC109-H、EC109-L细胞的转移潜能;利用RT-PCR和Westernblot方法,检测食管鳞癌高低转移细胞系EC9706-H、EC9706-L和EC109-H、EC109-L中RACK1的mRNA和蛋白表达水平。利用慢病毒转染技术上调RACK1低表达细胞中RACK1的表达后,采用Transwell实验检测其侵袭和迁移能力的变化。结果：EC9706-H细胞和EC109-H细胞的体外侵袭和迁移能力显著高于EC9706-L和EC109-L细胞系。RT-PCR和Westernblot实验结果显示,RACK1在EC9706-H细胞和EC109-H细胞中呈低表达;而在EC9706-L细胞和EC109-L细胞中呈高表达。通过慢病毒转染技术上调EC9706-H和EC109-H细胞系中RACK1的表达,二者的侵袭转移能力明显降低。结论：RACK1表达水平与食管鳞癌细胞的侵袭转移能力负相关,过表达RACK1能够抑制食管鳞癌细胞的侵袭转移能力。这提示,RACK1在食管鳞癌的侵袭转移中发挥重要作用,有可能成为其诊断、治疗及预后评估的新靶点。

英文摘要：

Objective： To explore the relationship between the expression of receptor for activated C kinase 1（ RACK1） and the invasive and metastatic potential of human esophageal squamous cell carcinoma（ ESCC） cell lines.Methods： RT- PCR and Western blot were used to examine the expression of RACK1 in ESCC cell lines. Lenti- virus infection technique was employed to up- regulate the expression of RACK1 in highly metastatic cells lines. Transwell assays were performed to identify the differences and changes of invasive and metastatic ability in ESCC cell lines. Results： The invasive and metastatic potentials of EC9706- H and EC109- H cells were higher than the matched EC9706- L and EC109- L cells. The expression levels of RACK1 in EC9706- H and EC109- H cells were lower than the matched EC9706- L and EC109- L cells. The invasive and metastatic potentials of EC9706- H and EC109- H cells were significantly decreased after overexpression of RACK1. Conclusion： The expression level of RACK1 may be inversely correlated to the invasive and metastatic behavior of ESCC cell lines and up- regulate the expression of RACK1 can inhibit the invasion and metastasis ability of ESCC.