中文摘要：

该文主要研究了Hela细胞对阳离子脂质体／DNA复合体的摄取机制。通过使用抑制剂抑制真核细胞跨膜运输的相关路径，再通过阳离子脂质体将绿色荧光蛋白基因、荧光素酶报告基因转染到细胞中。利用荧光显微镜、微光检测仪定性以及定量检测不同抑制剂浓度下转染效率的差异，MTT法检测各浓度下的细胞存活率，确定对相应路径的依赖性。结果显示，在细胞存活率保持在60％vX上的前提下，镜下观察到随着药物浓度的升高，荧光强度明显减弱，微光检测数据显示随着药物浓度增加，基因表达的效率具有明显的下降趋势。因此推测，Hela细胞主要通过网格蛋白以及小窝蛋白介导的路径摄取脂质体／DNA复合体，对于微管微丝介导的巨胞饮作用也具有一定的依赖性。

英文摘要：

The aim of this paper is to study the uptake mechanism for the cationic liposome/DNA complex by Hela cells. Eukaryotic cell membrane transport-related pathways were inhibited by inhibitors, and the gene with green fluorescent protein or luciferase was transfected into cells by cationic liposome. The transfection efficiency with different concentrations of inhibitors was detected by fluorescence microscope and low light level detector qualitatively or quantitatively. The MTT assay was used to detect the cell viability in order to evaluate the dependence on the pathways. The results suggested that when the cell viability remained at 60% or more, the fluorescence intensity significantly decreased and the efficiency of gene expression declined with the increase of the concentration of inhibition. This showed that the liposome/DNA complex was uptaken through the pathway which mediated by clathrin or caveolin and also through the phagocytosis that mediated by microtubule filaments.