中文摘要：

制动火箭指向病毒的向量到特定的房间通过修改鼠科的白血病病毒(MLV ) 的表面蛋白质被尝试，但是在许多情况中，蛋白质功能被影响，并且完成指向的交货是困难的。在这研究，我们试试制动火箭到基于的工程师 ecotropic Moloney 鼠科的白血病病毒( MoMLV )病毒的向量 totransduce hepatocytes.A 妄想的信封( Env )表示原生质标志被构造包含在 pgag-pol 的 Env.Following simultaneoustransfection 的N终点熔化到 aa+1 的肝炎 B 病毒 PreS2 肽， pLEGFP 和妄想的 env 原生质标志进 293T 细胞，有约 104 传染 units/ml 的 titer 的没有助手的 retrovirusstocks 在假类型向量显示出的 48 hpost-transfection.These 被完成制动火箭病毒的正常宿主范围，感染 hostNIH 3T3 细胞，尽管效率与带 wild-typeecotropic MoMLV 信封的 virions 的相比被减少。另外，结果的假类型病毒能没有 polymerized， transduce 人 hepatomacells 与那些 transductions 比较与相对高的乳头 ers 由 polymerized 人浆液白朊调停了人的浆液白朊。这条途径能有选择地习惯于 targethepatocytes。

英文摘要：

Targeting of retroviral vectors to specific cells was attempted through modifying the surface protein of the murine leukemia viruses （MLVs）, but in many cases the protein function was affected, and it is difficult to achieve the targeted delivery. In this study, we have tried to engineer ecotropic Moloney murine leukemia viruses （MoMLV）-based retroviral vectors to transduce hepatocytes. A chimeric envelope （Env） expression plasmid was constructed containing the hepatitis B virus PreS2 peptide fused to aa ＋1 at the Nterminus of Env. Following simultaneous transfection of pgag-pol, pLEGFP and chimeric env plasmids into 293T cells, helper-free retrovirus stocks with the titer of approximately 104 infectious units/ml were achieved at 48 h post-transfection. These pseudotype vectors showed the normal host range of retrovirus, infecting host NIH 3T3 cells, although the efficiency was reduced compared with that of virions carrying wild-type ecotropic MoMLV envelope. In addition, the resultant pseudotype viruses could transduce human hepatoma cells mediated by polymerized human serum albumin with relatively high titers in comparison with those transductions without polymerized human serum albumin. This approach can be used to target hepatocytes selectively.