中文摘要：

整合素是一类跨膜糖蛋白,能够与细胞外基质结合参与许多生物学过程,尤其是与癌细胞的增殖、迁移等密切相关.目前在研究膀胱癌的发生发展过程中,对整合素的关注较少.本研究利用稳定同位素标记技术（SILAC）蛋白质定量技术结合质谱分析技术,对3株不同转移潜能的膀胱癌细胞株中的蛋白质组差异表达进行研究,并通过蛋白质免疫印迹技术、实时荧光定量PCR、细胞免疫荧光照相和流式细胞术等多种技术进行验证.同时使用临床组织基因芯片结果对比分析表明,与正常膀胱表皮细胞HCV29相比,整合素α6在膀胱癌细胞中表达量均有明显的上升,且在非侵染性膀胱癌KK47细胞中的表达量最高,侵染性的膀胱癌YTS1细胞中表达量次之.这一结果说明整合素α6与膀胱癌的发展具有密切关系,并为深入研究整合素α6在膀胱癌发生发展中的作用提供了前期基础.

英文摘要：

The integrins,a family of transmembrane proteins,function in cell-to-cell and cell-to-extracellular matrix（ECM） adhesive interactions,and influence cell signaling of cell growth and differentiation.Expression of integrin α6 in three bladder cancer cell lines,HCV29,KK47 and YST1 were quantitatively analyzed by LC-MS using stable isotope labeling by amino acids in cell culture（SILAC）,a simple and powerful proteomic strategy.The results showed that the non-invasive bladder cancer cell line KK47 expressed the highest level of integrin α6.The expression of integrin α6 in invasive bladder cancer cell line YTS1 was also higher than in normal bladder epithelial cell line HCV29.Furthermore,these results were confirmed by Western blotting,q PCR,immunohistochemistry and flow cytometry.Clinical data of m RNA ITGA6 expression pattern from open-access database（www.oncomine.org） showed the same result during bladder cancer progression.All these indicated that integrin α6 is associated with the invasion progress of the bladder cancer.The preliminary data in this study may sparkle the fundamental role of integrin α6 in the research of bladder cancer.