新奇功能的基因 P12 从在区别媒介有教养的神经干细胞被孤立。充分, P12 基因的长度 cDNA 被克隆并且定序。结果证明编码 291 氨基酸的蛋白质包含一个开的读物框架。进一步,这基因被变成神经干细胞。功能的分析建议 pl2 蛋白质的表示密切与神经树突配置的区别被相关。另外,获得编码蛋白质, P12 顺序也在 Pichia pastoris 被表示酵母。关键词神经干细胞-区别-蛋白质表示-中国(资助号码 973-2006CB500702 )的国家基本研究节目支持的 Pichia pastoris 工程,中国(资助号码 30570590 )的国家自然科学基础,科技的上海市政的委员会的科学基础(资助Nos. 03JC1403 , 03JC14061 ),并且归还海外中国的学者,的科学研究基础州的教育部(资助没有。JWSL2003406 )
A novel fimctional gene P12 was isolated from neural stem cells cultured in differentiation medium. The fully length cDNA of P12 gene was cloned and sequenced. Result showed that it contains an open reading frame encoding a protein of 291 amino acids. Further, this gene was transferred into neural stem cell. Functional analysis suggests that the expression of p12 protein is closely correlated with differentiation of neural dendrite configuration. In addition, to obtain encoding protein, P12 sequence was also expressed in Pichia pastoris yeast.