在确定二价铁离子为A.f生长过程中惟一限制性底物争件下,通过考察初始亚铁离子浓度、初始pH值两种影响亚铁离子氧化代谢的主要因素来研究细菌的生长特性,得到以限制性底物亚铁离子浓度为表征的细菌生长曲线。利用基于Monod方程建立的细菌生长动力学方程模型,采用Matlab软件中的Gauss—Newton算法确定了在不同条件下细茵生长动力学参数,包括最大比生长速率μm、Monod常数K及Ro,推导出了不同条件下A.f对数期以底物Fe(Ⅱ)浓度为表征的生长动力学方程。
In order to explain kinetic behaviors of microorganisms in varied experiment conditions, growth kinetic model of Acidithiobacillus ferrooxidans ( A. f) was deduced based on Monod model. The kinetic parameters (Monod constant K, max specific growth rate μm, and Ro) of this model in different initial experiment condition were ob- tained by Gauss-Newton algorithm. The growth law in specific initial experiment condition and variable kinetic parameters were found. According to the growth behavior law studied under different initial experiment condition, the curves describing substrates (ferrous ion concentration) law and the growth velocity equations corresponding to the different initial experiment condition were obtained.