用玉米自交系组合R15×掖478的F2群体构建连锁图谱,并通过1年2点随机区组试验设计,考察玉米229个F2:4家系成株期的株高和穗位高。所建连锁图谱上共拟合146个SSR标记位点,覆盖基因组1666cM,标记间平均距离为11.4cM。用复合区间作图法进行QTL分析,共检测到8个控制株高的QTL,分别位于第2、3、4.5和8染色体;3个控制穗位高的QTL位点,位于第4染色体。单个株高QTL的贡献率变幅为6.67%-11.59%,单个穗位高QTL贡献率变幅为10.46%-12.15%。
A mapping population consisting of 229 F2 individuals derived from the cross between maize ( Zea mays L. ) inbreds R15 and Ye 478 was used in this study. Plant height (PH) and ear height (EH) of 229 F2:4 population individual plants were measured at two locations by random block design in 2003. A genetic linkage map was constructed containing 146 simple sequence repeat (SSR) markers, which covered 1 666 cM of the maize genome, with an average distance of 11.4 cM. Composite interval mapping identified eight QTL for PH located on chromosomes 2, 3, d, 5 and 8, and three QTL for EH located on chromosome 4, with explained phenotypic variation ranging from 6.67% to 11.59% and from 10.46% to 12.15%, respectively.