中文摘要：

目的构建氯化锂（LiCl）-匹罗卡品（pilocarpine，Pilo）诱导癫痫（epilepsy，EP）大鼠模型，给予不同浓度天麻素（gastrodin，Gas）预处理，探讨Gas在EP中的抗氧化作用及分子机理．方法清洁级雄性SD大鼠80只，随机分组，设对照组、模型组、Gas预处理60mg/kg、90mg/kg和120mg/kg组．构建EP大鼠模型，给予Gas预处理，观察大鼠EP发作过程，注射匹罗卡品3h后终止发作，24h后取材．检测EP发作后大鼠大脑皮质过氧化氢酶（catalase，CAT）、谷胱甘肽（glutathion，GSH）、超氧化物歧化酶（superoxide dismutase，SOD）和谷胱甘肽还原酶（glutathion reductase，GR）和总抗氧化能力（total antioxidation，T-AOC）含量变化，脂质氧化物丙二醛（malondialdehyde，MDA）含量变化，采用WesternBlot法检测有丝分裂原活化蛋白激酶家族（mitogen activated protein kinase family，MAPKs）的p38激酶（p38kinase，p38）的磷酸化水平．结果正常对照组无EP样发作行为；注射匹罗卡品后3h内，经Gas预处理的各组大鼠III级以上发作程度明显降低，与模型组相比差异有统计学意义（＜0.05）；模型组大鼠大脑皮质CAT、GSH、SOD、GR和T-AOC含量下降与对照组比较差异有统计学意义（＜0.05），MDA含量显著增加，与正常对照组比较差异有统计学意义（＜0.05）；不同浓度Gas预处理均能上调上述内源性巯醇抗氧化物的活性，减少脂质过氧化；模型组大鼠大脑皮质中有丝分裂原活化蛋白激酶家族p38的磷酸化水平明显高于正常对照组，而Gas预处理组p38磷酸化水平明显低于模型组（＜0.05）．结论Gas预处理可以减轻EP模型大鼠的发作程度，提高内源性抗氧化剂的含量，调控炎性信号通路中p38激酶活性，发挥神经保护作用．

英文摘要：

Objective To explo the antioxidant effect and molecular mechanism of gastrodin (Gas) in epilepsy(EP) rats induced by LiCl-pilocarpine(PILO) . Methods Eighty male SD rats were randomly divided into 5 groups: sham group, EP group, therapy groups(pretreated with 60 mg/kg, 90 mg/kg, 120 mg/kg of gastrodin respectively) . The EP model was esteblished by peritoneal injection of LiCl-PILO. Therapy groups were pretreated with various concerntration of Gas. The control group was given the same dosage of normal saline. The alteration of behavior was observed,the concentration of catalase(CAT),glutathion (GSH), superoxide dismutase(SOD),glutathion reductase(GR), total antioxidtion(T-AOC) and malondialdehyde(MDA) in rats brain cortex were detected by chemical colorimetric method, phosphorylation of p38 was determined by western blot. Results There was no EP seizure in sham group,and the EP seizure degree in therapy groups(gas pretreated groups) was significantly decreased,and had statistically significant difference with EP group( <0.05) . The EP model rats exhibited a significant decrease in the concentration of endogenous antioxidants(CAT, GSH, SOD,GR and T-AOC),while an increase of the concentration of MDA and phosphorylation p38 protein as compared to shamgroup( <0.05). After treatment of the Gas,treatment group rats attenuated the seizure degree,exhibited a significant increase of the concentration of endogenous antioxidants( <0.05),while a decrease in concentration of MDA and phosphorylation of p38 as compared to model group ( <0.05) . Conclusion Gas may have a neuroprotective role in central nervous system of epileptic rats modle by down-regulateing the seizure degree and the activity of p38 kinase and up-regulateing the content of endogenous antioxidants.