中文摘要：

目的：研究中药配伍对栀子苷在大鼠体内药动学的影响。方法：血浆样本采用70℃水浴法沉淀蛋白后进行HPLC分析,色谱条件：固定相为Dikma,Diamonsil,C18（5μm,150×4.6mm）;流动相为甲醇-0.2%乙酸（28：72,v/v）,流速：1ml/min;检测波长：240nm;以芍药苷为内标进行定量。结果：线性范围0.025-2.5μg/ml（r=0.9998）,最低检测浓度0.025μg/ml,回收率分别为97.60%,98%,99.2%;日内和日间精密度RSD均小于10%。大鼠灌胃栀子和柴芩承气汤后的药动学行为均符合一室模型,栀子苷在柴芩承气汤和单味栀子给药情况下药动学参数T1/2,Cmax和AUC0-24有明显差别（P〈0.05）。结论：栀子和柴芩承气汤复方在同一给药途径下,栀子苷在柴芩承气汤中比单味栀子的消除半衰期长,达峰时间长,消除速率慢,生物利用度比单味栀子给药高。

英文摘要：

Objective： To study the influence of compatibility of herbs on the pharmacokinetics of geniposide in rat plasma. Method ： Geniposide and an internal standard （ paeoniflorin ） in plasma sample were deproteinized with hot water bath at 70 ℃. The supernatant of treated plasma sample was detected directly by high performance liquid chromatography. Chromatographic conditions： solid phase is a reversed-phase Cls column; mobile phase is methanol-0.2% acetic acid （ 28： 72, v/v ） ; UV detective wavelength is 240 nm . Result： The calibration curve for geniposide was linear （ r = 0. 9998 ） in the concentration range 0. 025 - 2.5 μ/ml with a limit of detection at 0. 025 μg/ml. The recovery rate was respectively 97.60% ,98% ,99.2% ; The intra-day and inter-day precision of the geniposide were determined and their RSD did not exceed 10%. both plasma concentration-time curves are comformed to one-compartment model. T1/2 ,Cmax, AUC0-24 of the two group are significantly different when P 〈 0.05. Conclusion ： The results indicate that Chaiqinchengqi Decoction has delayed the elimination and increased the bioavailability of geniposide in rat plasma compared with Fructus Gardeniae.