中文摘要：

目的：比较腺苷酸环化酶（AC）和磷酸二酯酶（PDE）在活动力正常和活动力低下的人精子表达的差异。方法：收集活动力正常的人精子和a、b级精子低于20％的弱精子症患者的精子，提取总RNA，采用反转录．多聚酶链式反应（RT-PCR）的方法检测AC和PDE亚型mRNA的表达水平；采用酶联免疫吸附法测定两组样本环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）的含量。结果：与活动力正常的精子比较，可溶性腺苷酸环化酶（sAC）表达和cAMP含量在活动力低下的患者精子显著降低（P均〈0．01），而磷酸二酯酶4C（PDFAC）的表达显著增加（P〈0．01）；腺苷酸环化酶3（ACⅢ）的表达和cGMP的含量在活动力正常和活动力低下的精子差异无显著性（P〉0．05）。结论：精子sAC的表达减少和PDFAC的表达增加是精子活动力低下的原因之一。

英文摘要：

Objective： To compare the differences of expressions of adenylyl cyclase （AC） and phosphodiesterase （PDE） in ejaculated spermatozoa between healthy volunteers and the patients with asthenospermia. Methods： Ejaculated spermatozoa were collected from healthy volunteers and the patients with asthenospermia, Reverse transcription polymerase chain reaction （RT-PCR） was used to detect mRNA expression of AC and PDE subtypes in human spermatozoa. The concentrations of cAMP and cGMP in the samples were detected by enzyme-linked immunosorbent assay （ELISA）. Results ： Compared with healthy volunteers, expression of sAC mRNA and concentration of cAMP were significantly decreased in the patients with asthenospermia （ P 〈 0.01 ）, while the expression of PDFAC mRNA was significantly increased at the same time（P 〈0.01 ）. There were no marked differences in the expression of ACⅢ mRNA and concentration of cGMP between the two groups. Conclusion ： The sAC down-regulation and PDFAC up-regulation are possible reasons for asthenospermia. Natl J Androl,2006,12（3）：195-198