中文摘要：

目的：研究离体人肠道菌群对头花蓼提取物中槲皮素、槲皮苷和金丝桃苷3个单体成分在体外代谢转化的情况。方法：将3个成分与人肠道菌孵育液分别在37℃离体厌氧环境中培养24 h,采用超高压液相色谱-电喷雾-四级杆-飞行时间串联质谱仪进行检测,运用Metabolite Predict、Metabolite Detect对样品中的代谢产物进行预测并检测分析,结合Mass Defect Filter技术进一步有效提取代谢产物信息,排除基质背景干扰,并应用Data Analysis中Smart Formula功能推测代谢产物化学式。结果：槲皮苷和金丝桃苷主要以脱糖反应为主,苷元槲皮素进一步发生羟基化、甲基化、开环、还原等反应。结论：该实验建立了人肠道厌氧菌群体外代谢研究模型,为头花蓼提取物在人肠道内转化吸收提供了科学依据。

英文摘要：

Objective：To study the metabolism of the three components（quercetin,quercitrin and quercetin-3-O-galactoside） of effective fractions of Polygonum capitatum by human intestinal bacteria in vitro.Methods：Three components were added in the intestinal flora of human respectively,the sample were incubated under the anaerobic condition of 37 ℃ for 24 h.All the biological samples were detected by UPLC-Q-TOF/MS technology,Metabolite Predict and Metabolite Detect were used to predict and detect the metabolites,combined with Mass Defect Filter technology to remove the matrix interferences of the complex biological samples,and Smart Formula technology was used to surmise the formula of metabolites.Results：The main metabolic reaction of quercitrin and quercetin-3-O-galactoside were degalactoside,and then the major reaction of the aglycone quercetin were hydroxylation,methylation,open-loop and reduction.Conclusion：This method establishes a model for the study of human intestinal anaerobic bacteria in vitro,which provides the possible scientific evidence for three components from Polygonum capitatum biotransformation and absorption in human intestinal.