中文摘要：

目的探讨孕早期的雌、孕激素对小鼠调节性T细胞在体内的影响及其相关性。方法建立去势C57BL/6小鼠模型,2周后分别皮下注射雌激素、孕激素及雌孕激素联合重建小鼠性激素水平,模拟妊娠早期小鼠激素水平。维持2周后,用流式细胞术和免疫组化检测给药后小鼠外周血、脾脏、髂窝淋巴结、胸腺中调节性T细胞比例变化情况。结果实验结果显示,雌鼠去势后,单独的雌激素干预可明显提高脾脏、髂窝淋巴结、外周血CD＋4CD＋25Foxp3＋调节性T细胞在CD＋4T细胞中的比例,分别为（8.56±1.85）%,（7.63±1.68）%,（6.13±1.32）%;与溶剂组比较差异有显著性（P〈0.05）。但胸腺中调节性T细胞比例与溶剂组差异无显著性（P〉0.05）。单独妊娠早期水平的孕激素与溶剂组比较,调节性T细胞比例有所升高,但差异无显著性（P〉0.05）。而联合应用雌、孕激素对调节性T细胞的影响与单独应用雌激素的结果类似,两者差异无显著性。不同性别组给予相同干预药物,调节性T细胞的变化比例,雌雄两组差异无显著性（P〉0.05）。免疫组化也得到了类似的结果。结论单独应用相当于孕早期水平的雌激素可明显提高小鼠外周血,脾脏,髂窝淋巴结CD＋4CD＋25Foxp3＋调节性T细胞比例,而孕激素对外周调节性T细胞没有影响,两种激素对中枢淋巴器官的调节性T细胞均没有影响。小鼠T淋巴细胞中雌孕激素的受体分布及其功能与性别遗传没有相关性。

英文摘要：

Objective To investigate whether the estrogen（E2）,progesterone（P4） within the physiological concentration range of the early pregnancy could affect the expansion of CD＋4CD＋25 Foxp3＋ regulatory T cells. Methods We administrated pregnancy E2/P4 or saline in the ovariectomized and orchiectomized mice.After reconstituting the sex hormone to the physiological concentration range of the early pregnancy for two weeks,the mice were sacrificed,and lymphocytes were isolated from thymus,spleen,iliac lymph nodes and peripheral blood.The CD＋4CD＋25 Foxp3＋ regulatory T cells were detected by flow cytometry and immunohistochemistry. Results The groups received E2 alone showed a notable increase in the proportion of the Tregs that marked CD＋4CD＋25 Foxp3＋ in spleen,iliac lymph nodes and blood.CD＋4CD＋25 Foxp3＋ cells constituted（8.56±1.85）% of all CD＋4 cells in spleen,（7.63±1.68）% in iliac lymph nodes and（6.13±1.32）% in blood,While the proportion in the thymus could not be enhanced compared to the vehicle groups.The P4 administration could improve the proportion of Tregs lightly,but without statistical significance During the group combine with E2 and P4,each tissue samples showed a same trend in the increase of Tregs with E2 alone.Furthermore,no difference was found in the change of Tregs between the ovariectomized and orchiectomized mice.The similar results could be found in immunohistochemistry.Conclusion in vivo,E2 could drive the expansion of the Tregs in the secondary lymphoid organs and peripheral blood,but not P4.Neither of them could improve the proportion of Tregs in thymus.No gender difference could be found in the effect,which might be involved in the same distribution of hormone corelative receptors. Conclusion In vivo,E2 can drive the expansion of the Tregs in the secondary lymphoid organs and peripheral blood,but not P4.Neither of them can improve the proportion of Tregs in thymus.No gender difference could be found in the effect,which may involve in the same distributi