中文摘要：

象 CD4+Foxp3+ 规章的 T 房间一样的背景雌激素被显示不仅在维持母亲胎儿的忍耐而且对自体免疫的疾病有一个保护的角色。我们试图调查雌激素的怀孕层次是否是足够的导致移植忍耐至于维持我们建立了的胎儿母亲的 tolerance.Methods 在与雌激素重新组成的 C57BL/6 ovariectomized 老鼠的 H-Y 皮肤接枝移植。随后，连续每日的雌激素注射被管理。在外部血的 Tregs 和 cytokines 被结果显示了的流动 cytometry 和 ELISA pre-transplant.Results 和 post-transplant.Results 检测雌激素的怀孕层次能在第二等的淋巴的机关和外部血支持 Tregs (P < 0.05 ) 然而并非胸腺(P > 0.05 ) 。对待雌激素的接受者接受了 H-Y 皮肤接枝超过 35 天(中部的幸存时间(山区标准时间) ：（ 44.0 ? ш贅 ? 褤 ? 褤？？

英文摘要：

Background Estrogen as well as CD4＋Foxp3＋ regulatory T cells were shown to have a protective role not only in maintaining maternal-fetal tolerance but also against autoimmune diseases. We aimed to investigate whether the pregnancy levels of estrogen are enough to induce transplant tolerance as to maintain fetal-maternal tolerance. Methods We established H-Y skin graft transplantation in C57BL/6 ovariectomized mice that reconstituted with estrogen. Subsequently, consecutive daily estrogen injection was administrated. Tregs and the cytokines in the peripheral blood were detected by flow cytometry and ELISA pre- and post-transplant. Results The results indicated that pregnancy levels of estrogen could promote Tregs in secondary lymphoid organs and peripheral blood （P 〈0.05） but not thymus （P 〉0.05）. The estrogen-treated recipients accepted H-Y skin grafts for more than 35 days （median survival time （MST）： （44.0_＋1.2） days） compared with estrogen-untreated mice （MST： （23.0_＋1.6） days） （P 〈0.05）. It was also observed that estrogen up-regulated the expression of Foxp3, but did not affect CD3＋CD8＋ effector T-cells in non-transplant mice. While in the presence of H-Y antigens, the expression of Foxp3 was more significant and CD3＋CD8＋ effector T cells were decreased significantly （P 〈0.05）. Meanwhile, the up-regulated IL-10 and IL-4, and down-regulated IFN-v could be observed （P 〈0.05）. Conclusions Pregnancy levels of estrogen may promote the conversion of peripheral Tregs in secondary lymphoid organs, but show no effect on the natural Tregs production, differentiation and maturity in central lymphoid organs. Furthermore, pregnancy levels of estrogen could significantly prolong the survivals of H-Y skin grafts by the expansion of TreQs, suppression of CD3＋CD8＋ effector T-cells and immune shift towards Th2 cvtokines.