中文摘要：

目的克隆Ang-1基因，构建Ang-1／Pshuttle／Padeasy-1腺病毒表达载体，为进一步研究Ang-1防治卵巢癌病变的血管异质性提供理论基础。方法提取流产胎儿肝脏组织总RNA，RT—PCR扩增出Anal基因片段，并将其克隆到Pshuttle载体中进行序列分析，经PacⅠ线性化、连接到腺病毒表达载体Padeasy-1中，将Padeasy-1包装进脂质体、转染入人胚肾293细胞，测定病毒生物活性滴度。结果从流产胎儿肝脏组织总RNA中，扩增出1．4kb的cDNA片段，成功构建了Padeasy-1／Pshuttle／Ang-1表达载体。结论从流产胎儿肝脏组织中成功克隆Ang-1基因，成功构建Padeasy-1／Pshuttle／Ang-1表达载体，Padeasy-1／Pshuttle／Ang-1表达载体的滴度为1×10^11PFU／mL。

英文摘要：

Objective To clone angiopoietin-1 genes and construct Ang-1/Pshuttle/Pageasy-1 adenovirus expression vector, so as to make theory base for the further research on the Ang-1 prevention and cure blood vessel heterogenous of ovarian caner pathological changes. Methods Extracting mRNA from abortion embryo liver,amplified Ang-1 gene by RT-PCR and cloned into Pshuttle vector and sequenced,linearized by Pac Ⅰ and connected to adenovirus expression vector Padeasy-1, packaging Padeasy into liposome,transfect to 293 cells, determination of virus biological activity titer. Results From abortion embryo liver total RNA, RT- PCR amplified 1. 4kb cDNA fragment, successfully constructing Padeasy-1/Pshuttle/Ang-1 express vector. Conclusion Cloned Ang-1 gene and successfully constructing Padeasy-1/Pshuttle/Ang-1 express vector,it＇s titer is 1 × 10^11PFU/mL.