中文摘要：

为建立鸭疫里氏杆菌（RA）的血清学快速检测方法，本研究根据GenBank中登录的camp基因序列设计引物，以吉林RA分离株JL-1的基因组DNA为模板，通过PCR扩增camp基因，并构建pET-camp重组表达质粒。转化至E．coli BL21（DE3）中诱导表达出分子量约为37ku重组蛋白，western blot分析结果表明该蛋白能够与血清1、2、10、11和17型RA全茵体阳性血清发生特异性反应。以纯化的重组蛋白为包被抗原建立间接ELISA方法．结果显示其具有良好的特异性和重复性，与RA超声裂解抗原ELISA方法的符合率达到77．5％。研究结果进一步验证了Camp蛋白具有良好免疫原性，并且为RA多种血清型共同抗原，在RA的检测及亚单位疫苗开发中具有良好的应用价值。

英文摘要：

To establish the diagnosis method to detection of Riemerella. anafipestifer （RA） infection, the camp gene was amplified by PCR from genome of RA Jilin isolate with primers designed according to the sequence in GenBank, and cloned into pET28a vector for expressing in E.coli BL21 （DE3）. A molecular weight of about 37 ku recombinant protein was expressed by induction with IPTG and westemblot results show the protein was able to react withpositive sera of RA serotype 1, 2, 10, 11 and 17. Indirect ELISA method was established with purified Camp protein as coating antigen and proved to have good reproducibility and specificity. The coincidence rate with ELISA of RA ultrasound lysate antigen was 77.5%. This study further validates the Camp as RA serotypes common antigen has a higher value in the diagnosis and subunit vaccine development against RA.