中文摘要：

目的：探讨舌鳞状细胞癌中肿瘤干细胞对化疗的耐受作用。方法：用磁珠分选技术、细胞免疫荧光染色和药物毒性试验等方法，研究经分选后得到的肿瘤干细胞和阴性细胞在化疗药物作用后其细胞增殖活性的差异。结果：细胞免疫荧光结果显示，CD133和CD44在分选出来的CD133^＋／CD44^＋细胞高表达而在CD133^-／CD44^-细胞基本不表达。CCK8实验结果显示分选出来的CD133^＋／CD44^＋细胞较CD133^-／CD44^-细胞在化疗药物作用下有更高的细胞成活率。结论：经磁珠分选出来的舌癌肿瘤干细胞（CD133^＋／CD44^＋细胞）更能耐受化疗药物的作用。

英文摘要：

Objective： To investigate the chemotherapy-resistance of cancer stem cells in squamous cell cacinoma of tongue. Methods： CD133^＋/CD44^＋ and CD133^-/CD44^- subpopulation cells were sorted by magnetic cell sorting （MACS）. Single isolated CD133^＋/CD44^＋ and CD133-/CD44-cells were cultured in RPMI-1640 medium,containing basic fibroblast growth factor （bFGF, 10ng/mL）,epidermal growth factor （EGF, 20ng/mL）. Immunofluo- rescence was applied to detect the expression of CD133 and CD44 in these two subpopulation cells. CCK-8 assay was performed to analyze the drug sensitivity of the two subpopulation to anti-tumor drug,Cisplatin, 5-Fluoroura- cil and Vincristine. Result： Cell immunofluorescence showed that CD133 and CD44 could be detected in CD133^＋/ CD44^＋ cells while they could not be detected in CD133^-/CD44^- cells. Chemotherapeutic agents had a more obvious inhibitory effect on CD133^-/CD44^- cells than CD133^＋/CD44^＋ cells. Conclusion： The cancer stem cells in squamous cell cacinoma of tongue（CD133^＋/CD44^＋ cells） that sorted by MACS showed strong capability of tumor＇s re sistance to chemotherapeutic agents than CD133^-/CD44^- cells.