采用开顶式有机玻璃罩(OTCs)及紫外灯分别模拟气候变暖和紫外辐射B(uV—B)增强,对位于气候变暖和uV—B增强突出的青藏高原东缘、高山峡谷地云杉(Piceaasperata)幼苗的光合气体交换和叶绿素荧光参数进行测定分析,探讨云杉幼苗对气候变暖和uV—B增强的光合响应特性。结果显示:(1)UuB辐射增强显著抑制了云杉幼苗茎和根的伸长生长以及生物量的累积,显著降低了云杉幼苗的净光合速率(Pn)、最大光合速率(Pmax)和表观量子产量(圣),但是提高了光补偿点(LCP);uV—B辐射增强导致了云杉幼苗光合系统Ⅱ(PSII)的光抑制,使PSII有效量子产量(ФPSII)显著降低。(2)单纯OTC模拟增温显著提高了云杉幼苗的Pn和Pmax,而对气孔导度(Gy)、蒸腾速率(Tr)和中无显著影响。(3)模拟增温缓解了UV-B增强对云杉幼苗光合作用的抑制作用,显著提高了UV—B胁迫下幼苗的Pn Pmax PSII的潜在量子效率(Fv/Fm)和有效量子产量(ФPSII),并且提高了uV—B胁迫下幼苗茎、根的生长以及生物量的累积。研究表明,在未来气候变暖和UV—B辐射增强同时存在时,气候变暖能够在一定程度上缓解uV—B增强对云杉林光合作用的抑制作用。
Using open-top Chambers(OTCs) and UV-lamps,we manipulated climate warming and enhanced UV-B radiation. The parameters of photosynthetic gas exchange and chlorophyll fluorescence of Picea as- perata seedlings in the eastern fringe of the Tibetan Plateau,where climate warming and enhanced UV-B radiation(UV-B) were severe,were measured to investigate its photosynthetic responses to the two worse- ning environmental factors. The results showed that: (1)Enhanced UV-B restrained the net photosynthetic rate (Pn), the maximum photosynthetic rate(Pin,x) and the apparent quantum yield(Ф). However,it increased the pho- tosynthetic light compensation point(LCP). In addition,enhanced UV-B induced photo-inhibition of the photosyn- thetic system II(PS Il) in P. asperata seedlings, and it reduced the effective quantum yield of PS II(ФPSII ). In the end,enhanced UV-B significantly inhibited shoot elongation, root growth and biomass accumulation. (2)OTCs warming significantly enhanced Pn and P but it had no effects on G ,Tr and Ф. (3)UV-B effects on pho- tosynthesis were amended by warming to some extent,which could be judging from the increased Pn ,P intrinsic effieiency(Fv/Fm) and ФPSII of PS II ,and the improved growth. In the end,warming could alleviate the harmful effects of enhanced UV-B on photosynthesis of P. asperata.