中文摘要：

目的从人胃癌细胞株MGC-803中分离侧群（sP）细胞，研究其生物学特征。方法利用流式细胞分选术（FACS）从MGC-803细胞株中分选出sP细胞和非sP细胞亚群进行培养，采用克隆形成实验比较两组亚群细胞的体外增殖能力，NOD／SCID鼠成瘤实验检测两组亚群细胞体内成瘤能力。结果FACS结果显示，细胞株MGC-803中sP细胞亚群占总细胞的0．3％-1．2％；SP细胞体外克隆形成率为（0．862±0．050）％，非sP细胞体外克隆形成率为（0．325±0．207）％，两者比较P〈0．05；SP细胞最低成瘤数量是1×10。／只，非sP细胞为5×10^3%。结论人胃痛细胞株MGC．803中存在SP纲晌．萁毕物掌特忡与千纲晌甚本符合．

英文摘要：

To investigate isolation and biologic characteristics of the side population（SP） in human gas- tric cancer cell lines MGC-803. Methods Fluoresence activated cell sorting was used to sort SP and NSP cells from MGC- 803s. Tumorigenicity of the two subpopulations was observed by sphere forming assay. Proliferation was determined by sub-cutaneous tumor formation in NOD/SCID mice. Results The percent of SP cells was 0.3% -1.2%. Clone formation effi-ciency of SP cells and NSP ceils was （ 0. 862 ± 0.050 ） % and （ 0. 325 ±0. 207 ） % respectively, there were significant differences （ P 〈 0.05 ）. In vivo, the tumorigenic ability of SP cells was higher than that of NSP cells. Tumor formed only by 1 × 10^3 SP cells in nude mice, but 5 × 103 NSP cells. Conclusion Human gastric cancer cell lines MGC-803 contained some SP cells with stem cell properties.