中文摘要：

呼吸熵（RQ）是动物生理及能量代谢的常用指标之一．在计算呼吸熵时，释放CO2的量通常被直接应用．在具有钙化作用的海洋生物中，钙化会影响试验水体溶解无机碳（DIC）含量，如果被忽略可能会产生方法上的误差．本文通过呼吸瓶法对养殖长牡蛎及其3种附着生物（紫贻贝、玻璃海鞘和柄海鞘）呼吸熵与氧氮比（O／N）的测定，探讨水产动物呼吸熵测定中钙化作用的影响．结果表明：长牡蛎与紫贻贝的钙化率分别为（56．37±14．85）和（17．95±7．21）μmol·g^-1·h^-1，并因此减少水体DIC（3．72±0．80）和（1．48±0．14）mg·L^-1，分别占到呼吸增加DIC的（60．9±7．6）％和（39．9±5．7）％．4种试验生物的呼吸熵分别为：长牡蛎1．38±0．19、紫贻贝1．18±0．11、玻璃海鞘1．11±0．05、柄海鞘1．32±0．19．除玻璃海鞘外，均与O／N的结果相符．而其中具有钙化作用的长牡蛎和紫贻贝校正前的呼吸熵仅为0．56±0．19和0．70±0．04。不符合O／N的测定值．表明生物钙化对水体中的DIC有明显地吸收固定作用，在呼吸熵的测定中应被准确计算在内．

英文摘要：

Respiratory quotient （RQ） is one of the basic indices in physiology and energy metabo- lism of animals. When RQ is calculated, the amount of released CO2 is typically used directly. But for calcifying marine organisms, calcification which can affect dissolved inorganic carbon （DIC） content in the water may cause methodological error to some extent, if it is ignored. In this paper, RQ and O/N of cultured oyster Crassostrea gigas and 3 marine fouling animal species （Mytilus edu- lis, Ciona intestinalis, Styela clava） were measured in the respiratory chamber to discuss the effect of calcification in RQ determination. The results demonstrated that calcification rates of C. gigas and M. edulis were （56.37±14.85） and （17.95±7.21） μmol · g^-1 , h^-1, respectively. （3.72± 0.80） and （1.48±0.14） mg· L^-1 DIC in the water were correspondingly decreased, which occu- pied about （60.9±7.6） % and （39.9 ±5.7） % of respired CO2, respectively. RQ values of 4 ani- mals were C. gigas 1.38±0.19, M. edulis 1.18±0.11, C. intestinalis 1.11±0.05 and S. clava 1.32±0.19, which agreed with the O/N values except C. intestinalis. Meanwhile, the uncorrected RQ values of C. gigas and M. edulis were 0.56±0.19 and 0. 70±0. 04, respectively, which were contrary to the O/N values. Therefore, it was obviously that calcification could result in a signifi-cant influence on the respiratory quotient by affecting water DIC concentration and should be accu- rately calculated in RQ measurement.