中文摘要：

目的探讨抑癌基因蛋白merlin在青海回族胃癌组织中的表达特点。同时研究merlin蛋白对胃癌细胞增殖的影响。方法用免疫组化法检测青海回族47例胃癌组织、41例正常胃黏膜组织中merlin蛋白表达水平，利用PCR及9n,4序法分析外显子突变情况、用M1Tr法检测merlin对胃癌细胞增殖的影响。结果merlin蛋白在正常胃黏膜组织中表达较低（21．9％），而在胃癌组织中表达较高（74．5％），两者比较具有明显差异性（P〈0．05）。此外merlin蛋白在不同分化程度胃癌中阳性表达率分别为高、中分化组42．3％，低分化组76．2％，统计分析显示，merlin蛋白表达与胃癌分化程度、淋巴结转移及组织学分型情况有关（P〈0．05）。在对胃癌组织merlin基因外显子3、4、9、11和13的测序分析中发现，外显子3和4均有突变情况，主要突变类型为移码突变。外显子3和4突变率合计为23．4％。转染merlin后发现胃癌细胞增殖受到抑制。结论merlin蛋白表达与青海回族胃癌易感性有关，且有可能对细胞增殖有一定调节作用。

英文摘要：

Objective To investigate the expression of tumor suppressor merlin in gastric cancer of Hui （mus- lim） patients in Qinghai province, and to study the influences of merlin on cellular proliferation in gastric cancer cell line. Method Immunohistochemical staining was used to analyze the expression of merlin protein and the mutation of exons were analyzed by PCR and DNA sequencing in gastric cancer. MTT assayis used to detect cell proliferation in gastric cancer cell line. Results The expression of merlin protein is much lower （21.6%）in normal gastric mucosa, and it was higher in gastric cancer （74.5 % ）, the expression was significantly different between normal tissue and gastric cancer tissue（ P 〈 0.05 ）. The positive rate of merlin protein expression in high/moderate and low differentia- tion group were 42.3% and 76.2% respectively, merlin expression was related with tumor differentiation, lymph node metastasis, and histological types（P 〈 0.05 ）. PCR and DNA sequencing have found that exon 3 and 4 shown insertion or deletion mutation after detection of exon 3,4,9,11 and 13 of NF2 gene, the main mutation type belong to frameshift. The mutation percentage of exon 3 and 4 account for 23.4%. The gastric cancer cell proliferation is decreased after transfection merlin. Conclusion Merlin protein expression is related with gastric cancer susceptibility of Qinghai Hui （muslim） - Chinese, and merlin is involved in the proliferation of gastric cancer cell as well.