中文摘要：

目的建立金顶侧耳中烟酸含量的测定方法。方法采用RP-HPLC法,色谱柱为C18柱,流动相为甲醇-0.004mol·L-1庚烷磺酸钠-乙酸（5：95：0.2）,检测波长为263nm。结果烟酸对照品0.08～0.40μg与峰面积呈良好的线性关系（r=0.9999,n=5）,样品的平均回收率为103.84%,RSD=2.80%（n=9）。结论所建方法简便、准确、重复性好,可测定金顶侧耳中烟酸的含量。

英文摘要：

OBJECTIVE To establish an HPLC method for the determination of nicotinic acid in Pleurotm citrinopileatus Sing..METHODS Ionpair reversed-phase（RP） high performance liquid chromatography（HPLC）was performed using a C18 column with methanol-0.004 mol·L-1 heptane mahogany acid sodium-acetic acid（5：95：0.2）as mobile phase.The detection wavelength was set at 263 nm.RESULTS The calibration curve of nicotinic acid was linear from 0.08 to 0.4 μg（r = 0.9999）.The average recovery was 103.84% with RSD of 2.80%.CONCLUSION This method was proved to be accurate and simple.And it can be selected to control the quality of Pleurotm citrinopileatus Sing.