中文摘要：

目的采用反相HPLC法同时测定广金钱草中vicenin-1、夏佛塔苷和vicenin-3的含量。方法采用KromasilRP—C，。色谱柱（250mm×4．6mm，5肛m），柱温30℃，流动相A相为水-0．1％甲酸水溶液、B相为甲醇，梯度洗脱：0—8min、30％-32％B，8～32min、32％～34％B，32～60min、34％～40％B，流速1．0mL·min-1，检测波长272nm。结果vicenin-1在0．230—1．380p,g（r=0．9998）、夏佛塔苷在0．702～1．872p,g（r：0．9998）、vicenin-3在0．220—1．320P,g（r=0．9999）的线性关系良好；平均回收率（rt=6）分别为99．44％、99．58％、100．01％。结论所用方法具有简便、快速准确、重复性好的特点，可为广金钱草药材的质量控制提供依据。

英文摘要：

OBJECTIVE To establish an RP - HPLC method for simultaneous determination of vicenin - 1, schafloside and vicenin -3 in Desmoclium styracifolium. METHODS The determination was carried out on a Kromasil RP - C lS column （250 mm x 4. 6 mm,5 p~m ） with the column temperature set at 30 ~C. The mobile phase consisted of 0.1% aqueous formic acid （A） and metha- nol（B）. A gradient program was used according to the following profile：0 -8 min,30% -32% B;8 -32 rain,32% -34% B;32 -60 min,34% -40% B. The detection was carried out at 272 nm,and the flow rate was kept at 1.0 mL-min-l. RESULTS The method showed a good linear relationship within the range of 0.230 - 1. 380 p~g for vicenin - 1 （ r = 0. 9998 ）, 0. 702 - 1. 872 ixg for schaftoside （ r =0.9998 ） and 0.220 - 1. 320 g for vicenin - 3 （ r = 0. 9999 ） ; the average recovery was 99.44% for vicenin - 1, 99.58% for schaftoside and 100.01% for vicenin - 3. CONCLUSION The method is simple, rapid and accurate with good reproducibility, which provides the basis for quality control of Desmodium styracifolium.