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中文摘要：
目前,由食源性致病微生物引发的食品安全问题备受社会的关注.基于特征基因识别的食源性致病微生物的检测方法,由于其识别对象在体外具有稳定且易于扩增等优点,因而特异性和灵敏度较好,其应用范围也越来越广泛.本文综述了近些年基于特征基因识别检测食源性致病微生物的新方法,总结了作者研究小组围绕电化学发光和微流控技术在开展快速检测食源性致病微生物工作中取得的新进展,最后对此类技术的发展方向进行了展望.
英文摘要：
Recent years, the problems of food safety caused by foodborne pathogens have attracted a great deal of attention. For the detecting objects are stable and easy to amplify in vitro, gene-sensing techniques have been widely used to specifically, sensitively and rapidly detect foodborne pathogens. This paper summarizes the research advances on rapid detection of foodbome pathogens based on gene-sensing methods. Then, we present our novel results in foodborne pathogens detection using the electrochemiluminescence and microfluidic techniques. Finally, we discuss the future of gene-sensing approaches for rapid detection foodborne pathogens.
同期刊论文项目
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Miniaturized Paper-BasedGene Sensor for Rapid andSensitive Identification of Contagious Plant Virus,
Target-Triggered Enzyme-Free Amplification Strategy for Sensitive Detection of MicroRNA in Tumor Cel
Rapid and sensitive electrochemiluminescence detection of rotavirus by magnetic primer based reverse
An isothermal and sensitive nucleic acids assay by target sequence recycled rolling circle amplifica
Rapid and sensitive detection of Vibrio parahaemolyticus in sea foods by electrochemiluminescence po
Magnetic beads based rolling circle amplification-electrochemiluminescence assay for highly sensitiv
Sensitive and Homogeneous Protein Detection Based on Target-Triggered Aptamer Hairpin Switch and Nic
An electrochemiluminescent assay for high sensitive detection of mercury (II) based on isothermal ro
Integrated microfluidic reverse transcription-polymerase chain reaction for rapid detection of food-
Ultrasensitive and selective detection of mercury(II) in aqueous solution by polymerase assisted flu
Highly sensitive identification of foodborne pathogenic Listeria monocytogenes using single-phase co
Label-Free Detection of MicroRNA: Two-Step Signal Enhancement with a Hairpin-Probe-Based Graphene Fl
Rapid colorimetric gene-sensing of food pathogenic bacteria using biomodification-free gold nanopart
Highly sensitive protein detection based on aptamer probe and isothermal nicking enzyme assisted flu
Rapid and highly sensitive detection of mercury ion (Hg(2+)) by magnetic beads-based electrochemilum
A new kind of aptamer-based immunomagnetic electrochemiluminescence assay for quantitative detection
Sensitive colorimetric detection of Listeria monocytogenes based on isothermal gene amplification an
Amplified electrochemiluminescence detection of nucleic acids by hairpin probe-based isothermal ampl
Ultrasensitive aptamer-based bio bar code immunomagnetic separation and electrochemiluminescence met
One-Step Homogeneous Protein Detection Based on Aptamer Probe and Fluorescence Cross-Correlation Spe
Sensitive and isothermal electrochemiluminescence gene-sensing of Listeria monocytogenes with hyperb
Highly sensitive detection of protein and small molecules based on aptamer-modified electrochemilumi
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