为探讨水.水循环对生姜叶片的光保护作用,通过对生姜采取叶面喷施10mmol·L-1碘乙酰胺(IA)与遮光50%交互处理,研究了自然强光+清水(T1)、遮光50%+清水(T2)、自然强光+10mmol·L-1IA(T3)、遮光50%+10mmol·L-1 IA(T4)对生姜叶片叶绿素荧光参数、Mehler反应及超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)活性的影响.结果表明:处理过程中,T3、T4处理生姜叶片光合速率及最大光化学效率均持续降低,至处理9d时,光合速率分别较处理前降低了64%和33.2%,最大光化学效率分别降低了16.5%和10.9%,Me—hler反应分别升高了139.4%和72.6%,且SOD和APX活性显著增强,而T1、T2则无显著变化.处理6d时,一天内各处理生姜叶片光合速率、最大光化学效率在午间均显著降低,叶片的Mehler反应及SOD、APX活性在午间均显著增强,但以T3变幅较大,T4次之,T1、T2较小.可见,外源IA抑制生姜叶片光合速率后,Mehler反应及活性氧清除酶活性增强,表明水一水循环在耗散生姜叶片过剩光能中起重要作用.
To investigate the photo-protection of water-water circulation in ginger leaves, the effects of different treatments such as natural light + water control ( T1 ) , shading 50% + water control (T2), natural light + 10 mmol . L-1 IA (T3), shading 50% + 10 mmol. L-1 IA (T4) on leaf chlorophyll fluorescence parameters, Mehler reaction and the activities of SOD, APX of potted gin- ger were studied. The results showed that the Pn and Fv/Fm of ginger leaves in T3 and T4 reduced constantly, but that of T1 and T2 had no significant change during treatment. For example, at the ninth day after treatment, the Pn of T3 and W4 decreased by 64% and 33.2% respectively, and the Fv/Fm decreased by 16.5% and 10.9% respectively, while Mehler reaction of T3 and T4 increased by 139.4% and 72.6% respectively, the activity of SOD and APX were significantly higher than those of the controls. At the sixth day after treatment, the leaf Pn and Fv/Fm in all treatments re- duced significantly at noon, but Mehler reaction and the activities of SOD and APX increased markedly, and the largest amplitude was observed in T3 , followed by T4, and then T1 and T2. So, it was clear that Mehler reaction and the activity of reactive-oxygen scavenging enzymes increased when leaf P. was inhibited by exogenous IA. played an essential role in dissipating excessive The results suggested that water-water circulation light energy of ginger leaves.