为探讨外源ABA调控姜干旱胁迫的生理机制,以‘莱芜大姜’为试材,采用砂培,通过模拟干旱(5%PEG)和根系外施ABA(0.05 mmol·L(-1)),研究ABA对姜叶片活性氧代谢的影响。结果表明,外源ABA显著增加了姜叶片内源ABA含量,且以干旱胁迫下增加量为最多;同时,外源ABA亦有利于保持姜叶片较高的相对含水量。姜根系外施ABA早期,植株叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)及过氧化氢酶(CAT)活性均增强,进而显著降低了叶片中超氧阴离子()产生速率及过氧化氢(H_2O_2)含量,延缓了膜脂过氧化产物丙二醛(MDA)含量的增加;虽外源ABA处理后期,随干旱胁迫时间的延长,外施ABA处理植株叶片抗氧化酶活性有所降低,但其活性氧水平及MDA含量仍显著低于单一的干旱胁迫处理。表明外施0.05 mmol·L(-1)ABA有利于维持姜叶片活性氧代谢的正常进行,降低膜脂过氧化水平,增强植株抗干旱能力。
To investigate the physiological mechanism of exogenous abscisic acid(ABA)regulation of drought stress of ginger,the effects of active oxygen metabolism in ginger leaves applied ABA(0.05 mmol · L(-1))solution was studied with a ginger(Zingiber officinale Roscoe)cultivar‘Laiwu Ginger'by using the method of sand culture and 5% PEG simulating drought. The results showed that regardless of the water status,exogenous ABA significantly increased the content of endogenous ABA in ginger leaves,especially for the one under drought stress,meanwhile,exogenous ABA was found to be beneficial to holding relative water content in ginger leaves. At the early state of the application of exogenous ABA,the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)were enhanced markedly,which significantly reduced the . productivity rate and H_2O_2 content and delayed the increase of MDA content,at the last state of the application of exogenous ABA,although the Abstract. activity of antioxidant enzyme in ginger leaves of ABA treatment decreased with the prolongation of treatment time,the level of reactive oxygen of ABA treatment was still significantly lower than those under single drought stress,so was the MDA content. The results suggested that exogenous 0.05 mmol · L(-1) ABA is favorable to maintain the active oxygen metabolism and decrease the level of membrane lipid peroxidation,which enhance the ability of resisting drought stress in ginger leaves.