中文摘要：

利用生物信息学和RT-PCR方法从水稻幼苗组织中分离了1个新的C2H2型锌指蛋白基因RZF71,该基因编码一条250个氨基酸残基的多肽,含有两个典型的C2H2型锌指结构。半定量RT-PCR分析表明：RZF71在根、茎、叶和幼穗中呈组成性表达,在根中的表达丰度略高;在高盐和PEG6000胁迫的水稻幼苗组织中,RZF71的表达显著增强,但低温和ABA处理对该基因的表达量影响不大。农杆菌介导的洋葱表皮细胞GFP瞬时表达实验表明：RZF71定位于细胞核内。讨论了RZF71可能作为一个转录调控因子在水稻耐高盐和渗透胁迫中的作用。

英文摘要：

A rice zinc-finger protein gene, RZF71, encoding the C2H2-type zinc-finger transcription factor was isolated from rice （Oryza sativa L. subs. Japonica） by RT-PCR approach. Gene RZF71 encodes a 25 kDa protein with 250 amino acids, which contains two typical C2H2 zinc finger domains. The expression profiling showed that RZF71 was constitutively expressed in roots, culms, leaves, and flowering spikes. The semi-quantitative RT-PCR assay showed RZF71 was strongly induced by high-salinity and 20% PEG6000 treatments, but not regulated by low temperature and ABA （abscisic acid） treatments. Transient expression of the RZF71-GFP protein in onion epidermal cell showed that RZF71 was localized in cell nuclei. These results indicated that the RZF71 may play an important role in rice responses to salt and osmotic stresses as a transcription factor.