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超干处理对沙芥属蔬菜种子活力及抗氧化代谢的影响
  • ISSN号:0578-1752
  • 期刊名称:中国农业科学
  • 时间:2013.1.16
  • 页码:334-342
  • 分类:S662.3[农业科学—果树学;农业科学—园艺学]
  • 作者机构:[1]内蒙古农业大学农学院/内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室,呼和浩特010019
  • 相关基金:国家公益性行业(农业)科研专项(201203004); 国家自然科学基金(31160393,30860174,30260067); 内蒙古科技攻关项目(20050305,20060202); 内蒙古自然科学基金(2011BS0305,20080404MS0305)
  • 相关项目:蒙古高原特有属- - 沙芥属蔬菜种子脱水耐性机制的研究
中文摘要:

【目的】研究超干处理对沙芥属蔬菜种子活力及抗氧化代谢的影响,为沙芥属蔬菜种子耐干性及贮藏提供理论基础。【方法】采用硅胶干燥法将沙芥种子(4.5%)和斧形沙芥种子(4.3%)的含水量分别降至3.1%、2.2%、1.3%,测定不同含水量条件下种子的生活力及抗氧化代谢的变化。【结果】种子含水量由CK降至1.3%时,斧形沙芥种子活力保持较高水平,而沙芥种子则急剧下降;脱水后3个含水量的沙芥和斧形沙芥种子的相对电导率均低于CK,细胞膜完整性保持良好;超干处理会使沙芥属蔬菜种子的MDA含量和O2产生速率增加;超干处理对种子内4种抗氧化酶(SOD,CAT,GPX,APX)活性无明显影响。【结论】沙芥和斧形沙芥种子均具有较强的耐干性,但斧形沙芥种子抵御伤害的能力及种子的耐干性均强于沙芥种子,含水量2.2%是沙芥种子发芽率和活力的转折点。

英文摘要:

[Objective] The effect of ultradry treatment on dynamic change of vigor, antioxidative metabolism of Pugionium. seeds were determined, which would provide a theoretical basis for desiccation tolerance and storage of the seeds. [Method] The moisture content ofP. cornutum(L.) Gaertn. and P. dolabratum Maxim. were reduced to 3.1%, 2.2%, 1.3% from 4.5% and 4.3% (CK), respectively, with silica gel, and the vigor and antioxidative metabolism were detected when the seeds were re-moistened. [Result] The vigor of P. dolabratum Maxim. seeds maintained at a relatively higher level, while the P. cornutum(L.) Gaertn. seeds decreased sharply when the moisture content dropped to 1.3% from respective CK. Relative electrical conductivity of the two species seeds with ultradry treatment were lower than the CK and the integrity of cell membranes was conserved. Ultradry treatment accelerated the generation of superoxide generation rate (O~) and malondialdehyde (MDA), but the treatment did not affect the activities of antioxidant enzymes of the two species seeds obviously such as SOD, CAT, GPX, APX. [ Conclusion] The results showed that both of the seeds of P. cornutum(L,) Gaertn. and P. dolabratum Maxim. had strong desiccation tolerance, however, the ability of resisting damage and the desiccation tolerance ofP. dolabratum Maxim. seeds were stronger than P. cornutum(L.) Gaertn. seeds, and the moisture content of 2.2% was the turning point of germination and vigor of P. cornutum (L.) Gaertn. seeds.

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620