中文摘要：

目的了解糖尿病性大鼠阴茎海绵体平滑肌细胞（CCSM）凋亡和增殖特征,探讨其对糖尿病性大鼠CCSM数量的影响。方法利用链脲佐菌素建立糖尿病性大鼠模型。CCSM原代培养,并进行免疫细胞化学染色鉴定。采用WST-1检测细胞增殖率,采用流式细胞仪检测细胞凋亡率,采用qRT-PCR检测阴茎海绵体组织增殖细胞核抗原（PCNA）和凋亡蛋白caspase-3mRNA的表达。结果造模后12周,糖尿病组和正常对照组大鼠体质量和随机血糖水平差异显著（P〈0.001）。糖尿病组大鼠阴茎勃起功能较正常对照组大鼠差。原代培养所获细胞绝大部分为CCSM。糖尿病组CCSM的增殖速度明显低于正常对照组（P〈0.001）。在生长因子刺激下,糖尿病组CCSM增殖速率也慢于正常对照组（P〈O.05）。糖尿病组CCSM凋亡细胞个数及凋亡率均显著高于正常对照组（P〈0.001）。与正常对照组比较,糖尿病性大鼠阴茎海绵体组织中PCNAmRNA表达下降,而caspase-3 mRNA表达增强,差异显著（P〈0.001）。结论在长期糖尿病状态下,细胞凋亡增加与细胞增殖减慢并存,它们共同作用导致CCSM数量明显减少。

英文摘要：

Objective To explore the characteristics of cell apoptosis and proliferation of corpus cavernosum smooth muscle （CCSM） cells in diabetic rats.Methods From a SD rat model of diabetes induced by a single dose of streptozotocin,CCSM cells were isolated for primary culture and identified using immunocytochemical assays for SMα-actin.The proliferation of CCSM cells was evaluated by WST-1 assay,and flow cytometry was used to detect the cells apoptosis.Real-time fluorescence quantitative RT-PCR（qRT-PCR） was used to analyze the relative expression of proliferation cell nucleus antigen（PCNA） and caspase-3 mRNA.Results The proliferation rate of the primarily cultured CCSM cells from diabetic rats was significantly decreased and the apoptosis rate significantly increased compared with those of the cells from the control rats.The expression of PCNA mRNA was significantly lowered while caspase-3 mRNA significantly increased in the corpus cavernosum of the diabetic rats（P〈0.001）.Conclusion In rats with persisted hyperglycemia,a higher apoptosis rate and a lower proliferation rate both contribute to the reduction of CCSM cells.