中文摘要：

利用EcoRⅠ和HpaⅡ/MspⅠ双酶切建立适合于银杏基因组的甲基化敏感扩增多态性（methylation-sensitive amplification polymorphism,MSAP）分析体系,在全基因组水平检测银杏DNA甲基化修饰水平、模式及位点等表观遗传信息。结果显示,从54对MSAP选扩引物中,选出16对MSAP引物组合,共扩增产生454条清晰可辨且可重复的DNA条带,平均每对引物扩增获得28.38条带。在全部扩增位点中,检测到甲基化位点200个,CCGG/GGCC位点甲基化修饰比例为44%。部分银杏基因组甲基化修饰位点进行回收,最终分离了18条存在甲基化修饰的基因组DNA序列。BLASTn比对分析表明,银杏基因组中包括转录调控因子、反转录转座子、通道蛋白、启动子结合蛋白、蛋白激酶等在内的多种类型的DNA序列中均存在DNA甲基化修饰现象。

英文摘要：

To investigate the DNA methylated modification levels,patterns and sites of the Ginkgo biloba at the genome-wide level,the double digestion of EcoRⅠand HpaⅡ/MspⅠwas used to construct the Ginkgo biloba genomic MSAP（methylation-sensitive amplification polymorphism,MSAP）analysis system.By using 16 pairs of selective primers from the 54 pairs of MSAP selective primers,a total of 454 legible and repeatable amplified DNA bands were detected,on average 28.38 bands were observed after amplification with each primer pair.Two-hundred DNA methylated sites were detected among all the amplificated sites,which represented 44% ratio of methylated modification at CCGG/GGCC sites in Ginkgo biloba genome.Eighteen methylated DNA sequences were isolated and sequenced by extracting part of the amplificated sites.BLASTn comparison indicated that the DNA methylated modification phenomenon was existed in multiple types of DNA sequences,including repeated sequences,transcription regulators,retrotransposons,channel protein,promoter-binding protein,protein kinase,etc.