中文摘要：

目的：探讨移植脑源性神经营养因子（BDNF）体外诱导的骨髓间充质干细胞（BMSCs）治疗大鼠脑缺血模型的作用。方法：采用线栓法制作大脑中动脉闭塞（MCAO）模型，移植前6hBDNF体外诱导BMSCs分化为神经元样细胞。1×10^7经BD—NF体外诱导的或未诱导的BMSCs经鼠尾静脉移植。移植后第2，7，14，21，28天行大鼠神经缺失功能的测定，免疫组织化学染色和蛋白质印迹法检测神经细胞特异性烯醇化酶（NSE）的蛋白表达。结果：移植后第14天和第21天，BDNF-BMSCs移植组与BMSCs移植组较未移植组神经缺失功能评分明显好转，移植后第21天，BDNF-BMSCs移植组的神经功能缺失评分较BMSCs移植组高（P〈0．05）。BMSCs移植对运动功能的改善作用更明显，而在观察时间内BMSCs对感觉缺失功能恢复较慢。免疫组织化学染色和蛋白质印迹法显示移植第7天，NSE在BDNF-BMSCs移植组中的表达明显较其他组增强（P〈0．05）。结论：移植BDNF体外诱导的BMSCs能增强BMSCs改善缺血性大鼠神经缺失功能及NSE的蛋白表达。BDNF和BMSCs可能通过上调NSE表达改善缺血性大鼠神经缺失功能。

英文摘要：

Objective： To investigate the effect of bone mesenchymal stem cells （BMSCs） induced by brain- derived neurotrophic factor （BDNF） on neurological deficits in the rats with stroke. Methods： The BMSCs were harvested from 6-week-old male Sprague-Dawley rats. Adult male Sprague-Dawley rats weighing 250-280 g with transient middle cerebral artery occlusion were administered with 1-107 BMSCs or BMSCs induced by BDNF in 1 mL phosphate-buffered saline via tail vein at 48 h after stroke. All animals underwent neurological function test on days 2, 7, 14, 21 and 28. Immunohistochemical staining and Western blotting for neuron specific enolase （NSE） were applied. Results： The rats treated with BDNF-BMSCs and BMSCs showed significant improvement of neurological deficits compared to vehicle controls on days 21 and 28 （ P （0.05）, especially, BDNF-BMSCs exhibited remarkable functional recovery compared to BMSCs transplantation alone on day 21 （ P （0.05）. Both BDNF-BMSCs and BMSCs, especially BDNF-BMSCs, upregulated the NSE protein levels. Conclusion： BDNF and BMSCs might contribute to the functional improvement of neurological deficits partly via upregalating NSE expression level.